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Literature summary for 5.1.1.9 extracted from
- Detection and function of the intramolecular disulfide bond in arginine racemase: an enzyme with broad substrate specificity (2010), Chem. Biodivers., 7, 1591-1602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas taetrolens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C47A/C73A | the mutant displays about 7fold reduced specific activity compared to the wild type enzyme. The relative activities of C47A/C73AArgR for L-arginine, L-lysine, L-ornithine, D-arginine, D-lysine, and D-ornithine correspond well to those of wild type ArgR, but the relative activities for L-alanine and D-alanine increase to 25 and 26%, respectively | Pseudomonas taetrolens |
General Stability
| General Stability | Organism |
|---|---|
| the disulfide bond of ArgR is essential for ArgR to fold | Pseudomonas taetrolens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
L-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens |  |
| 1 | - |
D-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 1.2 | - |
L-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 1.2 | - |
D-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 1.8 | - |
L-lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 1.8 | - |
D-Lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 2.4 | - |
L-lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 2.4 | - |
D-Lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 3 | - |
L-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 3.1 | - |
D-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 3.6 | - |
D-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 3.7 | - |
L-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 8.4 | - |
L-alanine | mutant enzyme C74A/C73A ,in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 8.8 | - |
D-alanine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 15 | - |
D-alanine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 15.7 | - |
L-alanine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | an about 30times higher activity of ArgR is detected in the periplasm compared to that in the cytoplasm | Pseudomonas taetrolens | 5737 | - |
| periplasm | an about 30times higher activity of ArgR is detected in the periplasm compared to that in the cytoplasm | Pseudomonas taetrolens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000 | Pseudomonas taetrolens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas taetrolens | - |
- |
- |
| Pseudomonas taetrolens NBRC 3460 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas taetrolens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
periplasmic arginine racemase activity, mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens |
| 70.2 | - |
periplasmic arginine racemase activity, wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine | 11% activity compared to D-arginine | Pseudomonas taetrolens | L-alanine | - |
? | |
| D-alanine | 11% activity compared to D-arginine | Pseudomonas taetrolens NBRC 3460 | L-alanine | - |
? | |
| D-arginine | 100% activity | Pseudomonas taetrolens | L-arginine | - |
? | |
| D-arginine | 100% activity | Pseudomonas taetrolens NBRC 3460 | L-arginine | - |
? | |
| D-lysine | 103% activity compared to D-arginine | Pseudomonas taetrolens | L-lysine | - |
? | |
| D-ornithine | 42% activity compared to D-arginine | Pseudomonas taetrolens | L-ornithine | - |
? | |
| L-alanine | 12% activity compared to L-arginine | Pseudomonas taetrolens | D-alanine | - |
? | |
| L-alanine | 12% activity compared to L-arginine | Pseudomonas taetrolens NBRC 3460 | D-alanine | - |
? | |
| L-arginine | 100% activity | Pseudomonas taetrolens | D-arginine | - |
? | |
| L-arginine | 100% activity | Pseudomonas taetrolens NBRC 3460 | D-arginine | - |
? | |
| L-lysine | 104% activity compared to L-arginine | Pseudomonas taetrolens | D-lysine | - |
? | |
| L-Ornithine | 44% activity compared to L-arginine | Pseudomonas taetrolens | D-Ornithine | - |
? | |
| L-Ornithine | 44% activity compared to L-arginine | Pseudomonas taetrolens NBRC 3460 | D-Ornithine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 42000 | Pseudomonas taetrolens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArgR | - |
Pseudomonas taetrolens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
L-alanine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 2.6 | - |
D-alanine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 6.3 | - |
L-alanine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 6.8 | - |
D-alanine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 67.5 | - |
D-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 68.8 | - |
L-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 81.7 | - |
D-Lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 83.3 | - |
D-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 84.9 | - |
L-lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 87.7 | - |
L-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 93.5 | - |
L-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 94.6 | - |
D-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 104 | - |
D-Lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 109 | - |
L-lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 119 | - |
L-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 119 | - |
D-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Pseudomonas taetrolens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
L-alanine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0). 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 0.17 | - |
D-alanine | wild type enzyme,in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 0.74 | - |
L-alanine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 0.77 | - |
D-alanine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 22.1 | - |
D-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 22.9 | - |
L-ornithine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 23 | - |
D-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 23.4 | - |
L-ornithine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 43.7 | - |
D-Lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 45.1 | - |
D-Lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 46.2 | - |
L-lysine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 47.4 | - |
L-lysine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 92.6 | - |
L-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 94.6 | - |
D-arginine | mutant enzyme C74A/C73A, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 96 | - |
D-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
| 101 | - |
L-arginine | wild type enzyme, in 0.6 M CHES-NaOH buffer (pH 10.0), 0.1 mM pyridoxal 5'-phosphate, at 37°C | Pseudomonas taetrolens | |
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