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Literature summary for 5.1.1.7 extracted from
- Simple analytic method of diaminopimelate epimerase activity (2013), J. Biosci. Bioeng., 116, 253-255.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzme is an attractive target to facilitate development of antibacterial agents | Kitasatospora setae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dapF1, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS/pET15b | Kitasatospora setae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | inactivation by 50% acetone | Kitasatospora setae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| LL-2,6-Diaminoheptanedioate | Kitasatospora setae | - |
meso-Diaminoheptanedioate | - |
r | |
| LL-2,6-Diaminoheptanedioate | Kitasatospora setae KM-6054 | - |
meso-Diaminoheptanedioate | - |
r | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | inactivation by 50% acetone | Kitasatospora setae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kitasatospora setae | E4NI20 | gene dapF1; gene dapF1 | - |
| Kitasatospora setae KM-6054 | E4NI20 | gene dapF1; gene dapF1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS/pET15b by nickel affinity chromatography | Kitasatospora setae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.61 | - |
purified recombinant His-tagged enzyme, substrate meso-diaminoheptanedioate, pH 6.8, 30°C | Kitasatospora setae |
| 4.93 | - |
purified recombinant His-tagged enzyme, substrate LL-2,6-diaminoheptanedioate, pH 6.8, 30°C | Kitasatospora setae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| LL-2,6-Diaminoheptanedioate | - |
Kitasatospora setae | meso-Diaminoheptanedioate | - |
r | |
| LL-2,6-Diaminoheptanedioate | - |
Kitasatospora setae KM-6054 | meso-Diaminoheptanedioate | - |
r | |
| additional information | no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview | Kitasatospora setae | ? | - |
? | |
| additional information | no activity with DD-2,6-diaminoheptanedioate. Development of a simple method using thin-layer chromatography, in methanol/water (64:36) and with ninhydrin detection, and chiral column chromatography to allow preparation of pure diaminopimelate isomers and detect products, respectively, overview | Kitasatospora setae KM-6054 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAP epimerase | - |
Kitasatospora setae |
| DapF | - |
Kitasatospora setae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Kitasatospora setae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Kitasatospora setae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | diaminopimelate isomers are not only intermediates of the lysine biosynthesis diaminopimelate pathway but also essential components of bacterial peptidoglycan | Kitasatospora setae |
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