Literature summary for 5.1.1.7 extracted from

Usha, V.; Dover, L.G.; Roper, D.L.; Besra, G.S.
Characterization of Mycobacterium tuberculosis diaminopimelic acid epimerase: paired cysteine residues are crucial for racemization (2008), FEMS Microbiol. Lett., 280, 57-63.

Search for more literature for 5.1.1.7

Protein Variants

Protein Variants	Comment	Organism
C226A	complete loss of activity	Mycobacterium tuberculosis
C226S	severely compromised catalytic efficiency	Mycobacterium tuberculosis
C87A	complete loss of activity	Mycobacterium tuberculosis
C87S	severely compromised catalytic efficiency despite decrease in Km value	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
1,2-benzisothiazolidine 3-one	30 nM, complete inhibition	Mycobacterium tuberculosis
2-nitro-5-thiocyanatobenzoate	30 nM, complete inhibition	Mycobacterium tuberculosis
5,5'-dithiobis(2-nitrobenzoic acid)	30 nM, complete inhibition	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	meso-diaminoheptanedioate	mutant C87S, pH 7.5, 30°C	Mycobacterium tuberculosis
0.166	-	meso-diaminoheptanedioate	wild-type, pH 7.5, 30°C	Mycobacterium tuberculosis
0.215	-	meso-diaminoheptanedioate	mutant C226S, pH 7.5, 30°C	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WP19	-	-
Mycobacterium tuberculosis H37Rv	P9WP19	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
meso-diaminoheptanedioate	-	Mycobacterium tuberculosis	LL-2,6-diaminoheptanedioate	-	r
meso-diaminoheptanedioate	-	Mycobacterium tuberculosis H37Rv	LL-2,6-diaminoheptanedioate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.001	-	meso-diaminoheptanedioate	mutant C87S, pH 7.5, 30°C	Mycobacterium tuberculosis
0.0025	-	meso-diaminoheptanedioate	mutant C226S, pH 7.5, 30°C	Mycobacterium tuberculosis
0.1465	-	meso-diaminoheptanedioate	wild-type, pH 7.5, 30°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)