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Literature summary for 5.1.1.7 extracted from

  • Koo, C.W.; Sutherland, A.; Vederas, J.C.; Blanchard, J.S.
    Identification of active site cysteine residues that function as general bases: diaminopimelate epimerase (2000), J. Am. Chem. Soc., 122, 6122-6123.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
C217A mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen from L,L-3-fluoro-2,6-diaminopimelate, incapable of catalyzing HF elimination from D,L-3-fluoro-2,6-diaminopimelate Haemophilus influenzae
C217S catalyzes epimerization of L,L-diaminopimelate at 2% of the activity of the wild-type enzyme,catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate Haemophilus influenzae
C73A mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen. Mutant enzyme is able to rapidly catalyze elimination of the D,L-3-fluoro-2,6-diaminopimelate and is unable to catalyze elimination with the L,L-3-fluoro-2,6-diaminopimelate Haemophilus influenzae
C73S epimerization of L,L-diaminopimelate at 3% of the activity of the wild-type enzyme, catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate Haemophilus influenzae
C73S/C217S mutant enzyme is inactive as epimerase, slow elimination of HF from D,L-3-fluoro-2,6-diaminopimelate and L,L-3-fluoro-2,6-diaminopimelate Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,6S)-2,6-diaminoheptanedioate
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Haemophilus influenzae meso-diaminoheptanedioate
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?
DL-3-fluoro-2,6-diaminopimelic acid rapid elimination, enamine product is formed which spontaneously cyclizes to tetrahydrodipicolinic acid Haemophilus influenzae tetrahydrodipicolinic acid + HF
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?
LL-3-fluoro-2,6-diaminopimelic acid slow elimination of HF Haemophilus influenzae tetrahydrodipicolinic acid + HF
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?