Protein Variants | Comment | Organism |
---|---|---|
C217A | mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen from L,L-3-fluoro-2,6-diaminopimelate, incapable of catalyzing HF elimination from D,L-3-fluoro-2,6-diaminopimelate | Haemophilus influenzae |
C217S | catalyzes epimerization of L,L-diaminopimelate at 2% of the activity of the wild-type enzyme,catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate | Haemophilus influenzae |
C73A | mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen. Mutant enzyme is able to rapidly catalyze elimination of the D,L-3-fluoro-2,6-diaminopimelate and is unable to catalyze elimination with the L,L-3-fluoro-2,6-diaminopimelate | Haemophilus influenzae |
C73S | epimerization of L,L-diaminopimelate at 3% of the activity of the wild-type enzyme, catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate | Haemophilus influenzae |
C73S/C217S | mutant enzyme is inactive as epimerase, slow elimination of HF from D,L-3-fluoro-2,6-diaminopimelate and L,L-3-fluoro-2,6-diaminopimelate | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,6S)-2,6-diaminoheptanedioate | - |
Haemophilus influenzae | meso-diaminoheptanedioate | - |
? | |
DL-3-fluoro-2,6-diaminopimelic acid | rapid elimination, enamine product is formed which spontaneously cyclizes to tetrahydrodipicolinic acid | Haemophilus influenzae | tetrahydrodipicolinic acid + HF | - |
? | |
LL-3-fluoro-2,6-diaminopimelic acid | slow elimination of HF | Haemophilus influenzae | tetrahydrodipicolinic acid + HF | - |
? |