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Literature summary for 5.1.1.7 extracted from

  • Withe, P.J.; Lejeune, B.; Work, E.
    Assay and properties of diaminopimelate epimerase from Bacillus megaterium (1969), Biochem. J., 113, 589-601.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information pyridoxal phosphate does not stimulate Priestia megaterium
thiol compound required for activity Priestia megaterium
thiol compound 2,3-dimercaptopropan-1-ol is most effective Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
LL-diaminoheptanedioate at 37°C Priestia megaterium
100
-
meso-diaminoheptanedioate at 37°C Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
-
Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LL-2,6-Diaminoheptanedioate r, between 25°C and 45°C at pH 7.0, the equilibrium mixture contains 65% meso-isomer and 35% LL-isomer Priestia megaterium meso-Diaminoheptanedioate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 Priestia megaterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 in both directions Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 20% loss of activity Priestia megaterium
8.5
-
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 70% loss of activity Priestia megaterium