Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pyridoxal phosphate does not stimulate | Priestia megaterium | |
thiol compound | required for activity | Priestia megaterium | |
thiol compound | 2,3-dimercaptopropan-1-ol is most effective | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
LL-diaminoheptanedioate | at 37°C | Priestia megaterium | |
100 | - |
meso-diaminoheptanedioate | at 37°C | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
LL-2,6-Diaminoheptanedioate | r, between 25°C and 45°C at pH 7.0, the equilibrium mixture contains 65% meso-isomer and 35% LL-isomer | Priestia megaterium | meso-Diaminoheptanedioate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 | Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the relative rates of epimerization of LL-diaminoheptanedioate at 25°C, 37°C and 45°C are 0.77:1.00:1.15 | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | in both directions | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 20% loss of activity | Priestia megaterium |
8.5 | - |
2°C, overnight, 0.1 mM 2,3-dimercaptopropan-1-ol, 70% loss of activity | Priestia megaterium |