Application | Comment | Organism |
---|---|---|
molecular biology | the enzyme is valuable to serve as a novel non-antibiotic selectable marker in the generation of transgenic plants | Proteus mirabilis |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Proteus mirabilis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapor diffusion method, mixing of 2.5 mg/ml protein with 0.1 M sodium acetate, pH 4.6, 0.05 M lithium chloride, and 29% w/v PEG 8000, 20°C, X-ray diffraction structure determination and analysis at 1.74 A resolution | Proteus mirabilis |
Protein Variants | Comment | Organism |
---|---|---|
A165K/N174L/T391Y | site-directed mutagenesis, the mutant shows no activity towards L-Ala, L-Lys, or L-Arg | Proteus mirabilis |
N174L | site-directed mutagenesis, inactive mutant | Proteus mirabilis |
R173A | site-directed mutagenesis, inactive mutant | Proteus mirabilis |
R173K | site-directed mutagenesis, inactive mutant | Proteus mirabilis |
T391Y | site-directed mutagenesis, the mutant shows 47% reduced activity with L-lysine compared to the wild-type enzyme | Proteus mirabilis |
T391Y/S394Y | site-directed mutagenesis,the mutant exhibits significantly reduced specific activity towards L-Lys (6% residual activity) and toward L-Arg (0.9% residual activity) | Proteus mirabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.9 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis | |
21.8 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis | |
34.3 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis | |
37.6 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
47.7 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
75.5 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Proteus mirabilis | - |
D-lysine | - |
? | |
L-lysine | Proteus mirabilis BCRC10725 | - |
D-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | M4GGR9 | gene lyr | - |
Proteus mirabilis BCRC10725 | M4GGR9 | gene lyr | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Proteus mirabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | weaker activity | Proteus mirabilis | D-arginine | - |
? | |
L-arginine | weaker activity | Proteus mirabilis BCRC10725 | D-arginine | - |
? | |
L-lysine | - |
Proteus mirabilis | D-lysine | - |
? | |
L-lysine | preferred substrate | Proteus mirabilis | D-lysine | - |
? | |
L-lysine | - |
Proteus mirabilis BCRC10725 | D-lysine | - |
? | |
L-lysine | preferred substrate | Proteus mirabilis BCRC10725 | D-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lyr | - |
Proteus mirabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Proteus mirabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis | |
2.57 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis | |
10.17 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
10.83 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis | |
23.07 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
55.43 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Proteus mirabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on, cofactor binding residues of the enzyme are Thr245, Gly262, Tyr78, Gly263, Arg173, and Arg260, overview | Proteus mirabilis |
General Information | Comment | Organism |
---|---|---|
additional information | active site structure and substrate docking modeling, overview | Proteus mirabilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis | |
0.075 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y/S394Y | Proteus mirabilis | |
0.21 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
0.61 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged mutant T391Y | Proteus mirabilis | |
0.73 | - |
L-arginine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis | |
2.55 | - |
L-lysine | pH 9.0, 50°C, recombinant His-tagged wild-type enzyme | Proteus mirabilis |