Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acetoanaerobium sticklandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-carboxy-7-azoniabicyclo[2.2.1]heptane | a weak inhibitor | Acetoanaerobium sticklandii | |
7-azabicyclo[2.2.1]heptan-7-ium-1-carboxylate | a weak inhibitor of proline racemase, 29% inhibition at 142.5 mM | Acetoanaerobium sticklandii | |
additional information | substrate-product analogue inhibitors of racemases may only be effective when the active site is capacious and/or plastic, or when the inhibitor is sufficiently flexible | Acetoanaerobium sticklandii | |
tetrahydro-1H-pyrrolizine-7a(5H)-carboxylate | a bicyclic substrate-product analogue and noncompetitive inhibitor of proline racemase | Acetoanaerobium sticklandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
L-proline | pH and temperature not specified in the publication | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline | Acetoanaerobium sticklandii | - |
D-proline | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline | - |
Acetoanaerobium sticklandii | D-proline | - |
r | |
L-proline | two-base racemization mechanism via an aci-carboxylate intermediate | Acetoanaerobium sticklandii | D-proline | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not pyridoxal 5'-phosphate-dependent | Acetoanaerobium sticklandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
111 | - |
tetrahydro-1H-pyrrolizine-7a(5H)-carboxylate | pH and temperature not specified in the publication | Acetoanaerobium sticklandii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
67 | - |
pH and temperature not specified in the publication | Acetoanaerobium sticklandii | tetrahydro-1H-pyrrolizine-7a(5H)-carboxylate | |
349 | - |
pH and temperature not specified in the publication | Acetoanaerobium sticklandii | 1-carboxy-7-azoniabicyclo[2.2.1]heptane |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is a two-base racemase | Acetoanaerobium sticklandii |