Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.4 extracted from

  • Coutinho, L.; Ferreira, M.A.; Cosson, A.; Batista, M.M.; Batista, D.d.a..G.; Minoprio, P.; Degrave, W.M.; Berneman, A.; Soeiro Mde, N.
    Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection (2009), Mem. Inst. Oswaldo Cruz, 104, 1055-1062.
    View publication on PubMed
Search for more literature for 5.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
pyrrole-2-carboxylic acid inhibitor significantly affects parasite infection of Vero cells in vitro, inhibitor also hampers Trypanosoma cruzi intracellular differentiation, inhibitor reduces host cell invasion in Vero cells by Trypanososma cruzi in a dose-dependent manner, pre-treatment of the parasites with 1 mM of inhibitor does not lead to changes in their morphology and motility, but results in an up to 54% reduction in the percentage of parasitized cells and about 30% less parasites per cell when cultures are counted at day 4 after infection Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm TcPRAC localizes in the cytoplasm of intracellular amastigote forms of the parasite, near the flagellar pocket of the parasites Trypanosoma cruzi 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-proline Trypanosoma cruzi
-
 D-proline
-
 r

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-
metacyclic form
-
 Trypanosoma cruzi
-
trypomastigote clone F11-F5 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline
-
 Trypanosoma cruzi D-proline
-
 r

Synonyms

Synonyms Comment Organism
proline racemase
-
 Trypanosoma cruzi
TcPRAC
-
 Trypanosoma cruzi

Expression

Organism Comment Expression
Trypanosoma cruzi recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro additional information
Trypanosoma cruzi in amastigote forms of the parasites, the intensity of anti-TcPRAC labeling varies according to the time post infection reaching the highest signal of TcPRAC expression after 48 h, while the number of intracellular parasites increases by amastigote multiplication up

General Information

General Information Comment Organism
physiological function proline racemase is an effective mitogen for B cells, thus contributing to the parasites immune evasion and persistence in the human host Trypanosoma cruzi