BRENDA - Enzyme Database show
show all sequences of 5.1.1.4

Purification and mechanism of action of proline racemase

Cardinale, G.J.; Abeles, R.H.; Biochemistry 7, 3970-3978 (1968)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activates; optimal concentration: 0.02 M
Acetoanaerobium sticklandii
additional information
addition of pyridoxal 5'-phosphate does not increase activity
Acetoanaerobium sticklandii
NaBH4
activates
Acetoanaerobium sticklandii
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Acetoanaerobium sticklandii
2-Furoic acid
weak
Acetoanaerobium sticklandii
2-Thiophenecarboxylic acid
-
Acetoanaerobium sticklandii
alpha-Pipecoline
-
Acetoanaerobium sticklandii
Asp
-
Acetoanaerobium sticklandii
Fumaric acid
-
Acetoanaerobium sticklandii
HPO42-
no inhibition by H2PO4-
Acetoanaerobium sticklandii
iodoacetamide
-
Acetoanaerobium sticklandii
iodoacetate
DL-Pro and pyrrole-2-carboxylate can protect
Acetoanaerobium sticklandii
Maleic acid
-
Acetoanaerobium sticklandii
malonic acid
-
Acetoanaerobium sticklandii
Methoxyacetic acid
weak
Acetoanaerobium sticklandii
Phthalic acid
-
Acetoanaerobium sticklandii
pipecolic acid
weak
Acetoanaerobium sticklandii
pyrrole-2-carboxylic acid
-
Acetoanaerobium sticklandii
sarcosine
-
Acetoanaerobium sticklandii
succinic acid
-
Acetoanaerobium sticklandii
Tetrahydrofuroic acid
weak
Acetoanaerobium sticklandii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
L-Pro
-
Acetoanaerobium sticklandii
3.8
-
D-Pro
-
Acetoanaerobium sticklandii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetoanaerobium sticklandii
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
600
-
-
Acetoanaerobium sticklandii
Storage Stability
Storage Stability
Organism
stored 6 months in the refrigerator without significant loss of activity
Acetoanaerobium sticklandii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Pro
-
2122
Acetoanaerobium sticklandii
D-Pro
-
2122
Acetoanaerobium sticklandii
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
half-life: 3.6 min, in D2O 17 min
Acetoanaerobium sticklandii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activates; optimal concentration: 0.02 M
Acetoanaerobium sticklandii
additional information
addition of pyridoxal 5'-phosphate does not increase activity
Acetoanaerobium sticklandii
NaBH4
activates
Acetoanaerobium sticklandii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Acetoanaerobium sticklandii
2-Furoic acid
weak
Acetoanaerobium sticklandii
2-Thiophenecarboxylic acid
-
Acetoanaerobium sticklandii
alpha-Pipecoline
-
Acetoanaerobium sticklandii
Asp
-
Acetoanaerobium sticklandii
Fumaric acid
-
Acetoanaerobium sticklandii
HPO42-
no inhibition by H2PO4-
Acetoanaerobium sticklandii
iodoacetamide
-
Acetoanaerobium sticklandii
iodoacetate
DL-Pro and pyrrole-2-carboxylate can protect
Acetoanaerobium sticklandii
Maleic acid
-
Acetoanaerobium sticklandii
malonic acid
-
Acetoanaerobium sticklandii
Methoxyacetic acid
weak
Acetoanaerobium sticklandii
Phthalic acid
-
Acetoanaerobium sticklandii
pipecolic acid
weak
Acetoanaerobium sticklandii
pyrrole-2-carboxylic acid
-
Acetoanaerobium sticklandii
sarcosine
-
Acetoanaerobium sticklandii
succinic acid
-
Acetoanaerobium sticklandii
Tetrahydrofuroic acid
weak
Acetoanaerobium sticklandii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3
-
L-Pro
-
Acetoanaerobium sticklandii
3.8
-
D-Pro
-
Acetoanaerobium sticklandii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acetoanaerobium sticklandii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
600
-
-
Acetoanaerobium sticklandii
Storage Stability (protein specific)
Storage Stability
Organism
stored 6 months in the refrigerator without significant loss of activity
Acetoanaerobium sticklandii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Pro
-
2122
Acetoanaerobium sticklandii
D-Pro
-
2122
Acetoanaerobium sticklandii
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
half-life: 3.6 min, in D2O 17 min
Acetoanaerobium sticklandii
Other publictions for EC 5.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730678
Watanabe
Identification and characteriz ...
Clostridioides difficile, Ferroplasma acidarmanus, Haloarcula japonica, Haloarcula japonica DSM 6131, Thermococcus litoralis, Thermococcus litoralis DSM 5473
PLoS One
10
e0120349
2015
-
-
4
-
2
-
4
15
-
-
8
-
-
12
-
-
4
-
-
-
-
-
12
4
8
-
-
15
4
-
-
-
-
-
4
-
-
4
-
-
2
-
4
4
-
15
-
-
8
-
-
-
-
4
-
-
-
-
12
4
8
-
-
15
4
-
-
-
-
-
-
-
15
15
748829
Caballero
Phylogenetic and syntenic dat ...
no activity in Trypanosoma brucei, no activity in Trypanosoma congolense, Trypanosoma conorhini, Trypanosoma conorhini TCC025, Trypanosoma cruzi, Trypanosoma cruzi CL Brener, Trypanosoma cruzi marinkellei, Trypanosoma cruzi marinkellei TCC344, Trypanosoma dionisii, Trypanosoma dionisii TCC211, Trypanosoma erneyi, Trypanosoma erneyi TCC1946, Trypanosoma grayi, Trypanosoma grayi ANR4, Trypanosoma lewisi, Trypanosoma lewisi TCC034, Trypanosoma rangeli, Trypanosoma serpentis, Trypanosoma serpentis TCC1052, Trypanosoma sp., Trypanosoma sp. TCC1825 / RCF-2014, Trypanosoma sp. TCC339, Trypanosoma sp. TCC878, Trypanosoma sp. TCC878 RCF-2014, Trypanosoma vivax, Trypanosoma vivax Y486
Parasit. Vectors
8
222
2015
-
-
13
-
-
-
-
-
-
-
-
-
-
47
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
14
-
-
-
727156
Harty
Inhibition of serine and proli ...
Acetoanaerobium sticklandii
Bioorg. Med. Chem. Lett.
24
390-393
2014
-
-
1
-
-
-
4
1
-
-
-
1
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
2
-
-
1
1
-
-
-
2
4
1
1
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
727265
Wu
The Clostridium difficile prol ...
Clostridioides difficile
Can. J. Microbiol.
60
251-254
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
749144
Fikru
A proline racemase based PCR ...
Trypanosoma vivax, Trypanosoma vivax ILRAD 1392
PLoS ONE
9
e84819
2014
-
1
-
-
1
-
-
-
-
-
-
-
-
15
-
-
-
-
-
2
-
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-
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-
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1
-
-
-
1
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728600
Berneman
Combined approaches for drug d ...
Trypanosoma cruzi
PLoS ONE
8
e60955
2013
-
-
1
-
-
-
9
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
6
-
-
-
-
1
-
-
-
-
-
9
6
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
715163
Bryan
Genetic immunization converts ...
Trypanosoma cruzi, Trypanosoma cruzi Y
Infect. Immun.
78
810-822
2010
-
-
1
-
-
-
-
-
-
-
1
-
-
12
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704196
Rubinstein
Catalyzing racemizations in th ...
Trypanosoma cruzi
J. Am. Chem. Soc.
131
8513-8521
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
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1
1
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-
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-
-
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1
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-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705499
Coutinho
Inhibition of Trypanosoma cruz ...
Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
104
1055-1062
2009
-
-
-
-
-
-
1
-
1
-
-
1
-
3
-
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3
-
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1
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1
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1
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1
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3
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
705500
Coatnoan
Proline racemases: Insights in ...
Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
104
295-300
2009
-
-
1
-
-
-
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-
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2
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3
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2
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2
1
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2
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2
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4
-
-
2
2
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-
-
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-
1
1
2
2
-
-
705596
Chamond
Proline racemases are conserve ...
Trypanosoma vivax
Mol. Biochem. Parasitol.
165
170-179
2009
-
1
1
-
-
-
1
2
-
-
1
1
-
7
-
-
1
-
-
3
-
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1
-
1
-
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-
1
-
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-
1
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1
1
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1
1
2
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1
1
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1
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3
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
675621
Stenta
The Catalytic Activity of Prol ...
Trypanosoma cruzi
J. Phys. Chem. B
112
1057-1059
2008
-
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3
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2
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676749
Goytia
Molecular and structural discr ...
Clostridioides difficile, Clostridioides difficile VPI10463
PLoS ONE
2
e885
2007
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1
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1
2
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7
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2
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1
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1
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1
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2
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1
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1
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-
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-
676832
Buschiazzo
Crystal structure, catalytic m ...
Trypanosoma cruzi
Proc. Natl. Acad. Sci. USA
103
1705-1710
2006
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1
1
2
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1
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4
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1
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1
1
1
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2
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1
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1
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1
1
1
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-
662908
Chamond
Trypanosoma cruzi proline race ...
Trypanosoma cruzi
Mol. Microbiol.
58
46-60
2005
-
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-
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2
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1
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2
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1
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652377
Chamond
Biochemical characterization o ...
Trypanosoma cruzi
J. Biol. Chem.
278
15484-15494
2003
-
1
1
-
-
-
1
2
3
-
3
-
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6
-
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-
-
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5
3
1
1
-
1
-
2
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-
1
2
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-
1
1
1
-
-
-
-
1
2
2
3
-
3
-
-
-
-
-
-
-
-
5
3
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
653295
Reina-San-Martin
A B-cell mitogen from a pathog ...
Trypanosoma cruzi
Nat. Med.
6
890-897
2000
-
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1
-
-
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4
-
1
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1
1
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3
-
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1
1
1
-
1
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-
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-
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1
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1
-
-
-
-
-
4
-
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1
-
1
1
-
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-
-
-
-
-
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1
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
2125
Fisher
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2529-2537
1986
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1
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1
1
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1
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1
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1
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1
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1
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-
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-
-
2126
Fisher
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2543-2551
1986
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2127
Belasco
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2564-2571
1986
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2128
Fisher
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2538-2542
1986
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2129
Belasco
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2558-2564
1986
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2130
Albery
Energetics and mechanism of pr ...
Acetoanaerobium sticklandii
Biochemistry
25
2572-2577
1986
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2131
Belasco
Energetics of proline racemase ...
Acetoanaerobium sticklandii
Biochemistry
25
2552-2558
1986
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2124
Rudnick
Reaction mechanism and structu ...
Acetoanaerobium sticklandii
Biochemistry
14
4515-4522
1975
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3
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1
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2
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1
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1
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2123
Keenan
The inhibition of proline race ...
Acetoanaerobium sticklandii
Biochem. Biophys. Res. Commun.
57
500-504
1974
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2122
Cardinale
Purification and mechanism of ...
Acetoanaerobium sticklandii
Biochemistry
7
3970-3978
1968
3
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18
2
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1
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1
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3
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18
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2
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1
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1
1
1
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1
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2121
Stadtman
Studies on the enzymic reducti ...
Acetoanaerobium sticklandii
J. Biol. Chem.
228
983-997
1957
3
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3
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