Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor-independent enzyme | Limosilactobacillus fermentum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Limosilactobacillus fermentum |
General Stability | Organism |
---|---|
stabilized by presence of reduced thiols. After 3 h at 30°C, samples of the enzyme containing either dithiothreitol or Glu or both show about 80% retention of the original activity, whereas samples that lack both dithiothreitol and Glu retain less than 40% of the starting activity | Limosilactobacillus fermentum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Limosilactobacillus fermentum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
L-Glu | - |
Limosilactobacillus fermentum | |
0.33 | - |
D-Glu | - |
Limosilactobacillus fermentum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Limosilactobacillus fermentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus fermentum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
enzyme from Lactobacillus fermenti | Limosilactobacillus fermentum |
161 | - |
enzyme expressed in Escherichia coli | Limosilactobacillus fermentum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glu | - |
Limosilactobacillus fermentum | L-Glu | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Limosilactobacillus fermentum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
D-Glu | - |
Limosilactobacillus fermentum | |
76 | - |
L-Glu | - |
Limosilactobacillus fermentum |