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Literature summary for 5.1.1.3 extracted from

  • Choi, S.Y.; Esaki, N.; Yoshimura, T.; Soda, K.
    Overproduction of glutamate racemase of Pediococcus pentosaceus in Escherchia coli clone cells and its purification (1991), Protein Expr. Purif., 2, 90-93.
    View publication on PubMed
Search for more literature for 5.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pediococcus pentosaceus

General Stability

General Stability Organism
repeated thawing and melting of the enzyme causes inactivation Pediococcus pentosaceus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pediococcus pentosaceus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration Pediococcus pentosaceus
29140
-
 calculation from nucleotide sequence Pediococcus pentosaceus

Organism

Organism UniProt Comment Textmining
Pediococcus pentosaceus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pediococcus pentosaceus

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.5, 1 mM DL-GLu, 10% glycerol, 0.1% 2-mercaptoethanol, 0.1 mM phenylmethanesulfonyl fluoride, stable for several months Pediococcus pentosaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glu
-
 Pediococcus pentosaceus L-Glu
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Pediococcus pentosaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 25°C, 15 min, stable Pediococcus pentosaceus