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Literature summary for 5.1.1.3 extracted from
- Cloning and expression in Escherichia coli of the glutamate racemase gene from Pediococcus pentosaceus (1986), Agric. Biol. Chem., 50, 2823-2830.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pediococcus pentosaceus |
General Stability
| General Stability | Organism |
|---|---|
| irreversible loss of activity on storage at DL-Glu and a reducing thiol such as 2-mercaptoethanol. Inactivation may be due to air oxidation of a free thiol residue | Pediococcus pentosaceus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Nitro-5-thiocyanobenzoate | - |
Pediococcus pentosaceus |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Pediococcus pentosaceus | |
| hydroxylamine | no inactivation | Pediococcus pentosaceus | |
| additional information | no inactivation by carbonyl reagents such as hydroxylamine and NaBH4 | Pediococcus pentosaceus | |
| p-chloromercuribenzoate | - |
Pediococcus pentosaceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
D-Glu | - |
Pediococcus pentosaceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
HPLC gel filtration | Pediococcus pentosaceus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pediococcus pentosaceus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pediococcus pentosaceus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
- |
Pediococcus pentosaceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glu | - |
Pediococcus pentosaceus | L-Glu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Pediococcus pentosaceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pediococcus pentosaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pediococcus pentosaceus |
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Release 2023.1 (January 2023)
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