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Literature summary for 5.1.1.24 extracted from

  • Adachi, M.; Shimizu, R.; Kato, S.; Oikawa, T.
    The first identification and characterization of a histidine-specific amino acid racemase, histidine racemase from a lactic acid bacterium, Leuconostoc mesenteroides subsp. sake NBRC 102480 (2019), Amino Acids, 51, 331-343 .
    View publication on PubMed
Search for more literature for 5.1.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leuconostoc mesenteroides subsp. mesenteroides

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure in complex with pyridoxal 5'-phosphate at a 2.1 A resolution. Residues Phe44, His286, and Val357 may control the substrate specificity Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.14
-
 L-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
17.26
-
 D-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Leuconostoc mesenteroides subsp. mesenteroides
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-histidine Leuconostoc mesenteroides subsp. mesenteroides
-
 L-histidine
-
 r
D-histidine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 L-histidine
-
 r
L-histidine Leuconostoc mesenteroides subsp. mesenteroides
-
 D-histidine
-
 r
L-histidine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
 D-histidine
-
 r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides subsp. mesenteroides Q03Z32
-
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Q03Z32
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides L-histidine
-
 r
D-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 L-histidine
-
 r
L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides D-histidine
-
 ?
L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides D-histidine
-
 r
L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 D-histidine
-
 ?
L-histidine
-
 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 D-histidine
-
 r
additional information the enzyme is highly specific for histidine. No substrates: L-alanine, L-arginine, L-asparagine, L-glutamate, L-glutamine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, l-phenylalanine, L-proline, L-serine, L-threonine, L-tyrosine, or L-valine Leuconostoc mesenteroides subsp. mesenteroides ?
-
 ?
additional information the enzyme is highly specific for histidine. No substrates: L-alanine, L-arginine, L-asparagine, L-glutamate, L-glutamine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, l-phenylalanine, L-proline, L-serine, L-threonine, L-tyrosine, or L-valine Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, calculated from sequence Leuconostoc mesenteroides subsp. mesenteroides

Synonyms

Synonyms Comment Organism
LEUM_0422
-
 Leuconostoc mesenteroides subsp. mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Leuconostoc mesenteroides subsp. mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life 9 min Leuconostoc mesenteroides subsp. mesenteroides
45
-
 half-life 1.9 min Leuconostoc mesenteroides subsp. mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
96.9
-
 L-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides
185.4
-
 D-histidine pH 7.0, 30°C Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Leuconostoc mesenteroides subsp. mesenteroides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Leuconostoc mesenteroides subsp. mesenteroides