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Literature summary for 5.1.1.18 extracted from

  • Uda, K.; Abe, K.; Dehara, Y.; Mizobata, K.; Edashige, Y.; Nishimura, R.; Radkov, A.D.; Moe, L.A.
    Triple serine loop region regulates the aspartate racemase activity of the serine/aspartate racemase family (2017), Amino Acids, 49, 1743-1754 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene SRR, sequence comparisons and phylogenetic tree Mus musculus
sequence comparisons and phylogenetic tree Crassostrea gigas
sequence comparisons and phylogenetic tree Penaeus monodon
sequence comparisons and phylogenetic tree Acropora millepora

Protein Variants

Protein Variants Comment Organism
H150S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
H150S/N152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
H150S/P151S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
H150S/P151S/F152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Penaeus monodon
H150S/P151S/N152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
additional information introducing the triple serine loop region into SerRs promotes aspartate racemization Mus musculus
additional information introducing the triple serine loop region into SerRs promotes aspartate racemization Crassostrea gigas
additional information introducing the triple serine loop region into SerRs promotes aspartate racemization Penaeus monodon
additional information introducing the triple serine loop region into SerRs promotes aspartate racemization Acropora millepora
N152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
P150S/P151S/F152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Acropora millepora
P150S/P151S/Y152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Crassostrea gigas
P151S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus
P151S/F152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Acropora millepora
P151S/N152S site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Lineweaver-Burk kinetics Mus musculus
additional information
-
additional information Lineweaver-Burk kinetics Crassostrea gigas
additional information
-
additional information Lineweaver-Burk kinetics Penaeus monodon
additional information
-
additional information Lineweaver-Burk kinetics Acropora millepora
7.71
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
8.48
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
12.7
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
14.9
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
15.1
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
18
-
L-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
19.2
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
20.9
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
21.4
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
21.5
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
28.9
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
28.9
-
D-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus
29.3
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
30
-
L-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus
37.4
-
D-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
38.1
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
42.4
-
L-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
42.6
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
44.1
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
45.1
-
D-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
50.6
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
53.4
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
55.2
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
69.7
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
69.9
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
119
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
125
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon
146
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
172
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
252
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Mus musculus
-
D-serine
-
r
L-serine Crassostrea gigas
-
D-serine
-
r
L-serine Penaeus monodon
-
D-serine
-
r
L-serine Acropora millepora
-
D-serine
-
r

Organism

Organism UniProt Comment Textmining
Acropora millepora
-
-
-
Crassostrea gigas A0A0U5AKI6
-
-
Mus musculus Q9QZX7
-
-
Penaeus monodon A0A0U4MRI4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine
-
Mus musculus D-serine
-
r
L-serine
-
Crassostrea gigas D-serine
-
r
L-serine
-
Penaeus monodon D-serine
-
r
L-serine
-
Acropora millepora D-serine
-
r

Synonyms

Synonyms Comment Organism
SerR
-
Mus musculus
SerR
-
Crassostrea gigas
SerR
-
Penaeus monodon
SerR
-
Acropora millepora
SRR
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
-
-
assay at Penaeus monodon
30
-
assay at Mus musculus
30
-
assay at Crassostrea gigas
30
-
assay at Acropora millepora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00178
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
0.00211
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
0.00264
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
0.00366
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
0.00392
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
0.00423
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
0.00488
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
0.00772
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
0.00861
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
0.00871
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
0.0335
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon
0.0414
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
0.0551
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
0.057
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon
0.277
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
0.285
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.376
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
0.502
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
0.516
-
D-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
0.523
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
0.605
-
L-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
0.611
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
0.816
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
0.824
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
0.84
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.872
-
L-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
2.16
-
D-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
3.74
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
5.68
-
D-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus
5.71
-
L-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mus musculus
8
-
assay at Crassostrea gigas
8
-
assay at Penaeus monodon
8
-
assay at Acropora millepora

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mus musculus
pyridoxal 5'-phosphate
-
Crassostrea gigas
pyridoxal 5'-phosphate
-
Penaeus monodon
pyridoxal 5'-phosphate
-
Acropora millepora

General Information

General Information Comment Organism
evolution SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis Mus musculus
evolution SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity Crassostrea gigas
evolution SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity Penaeus monodon
evolution SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity Acropora millepora
additional information role of individual residues at position 150-152, overview Mus musculus
additional information role of individual residues at position 150-152, overview Crassostrea gigas
additional information role of individual residues at position 150-152, overview Penaeus monodon
additional information role of individual residues at position 150-152, overview Acropora millepora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0000559
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
0.0000651
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Penaeus monodon
0.000137
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
0.000138
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S Acropora millepora
0.000174
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
0.000205
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/F152S Acropora millepora
0.000324
-
L-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
0.000338
-
D-serine pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S Crassostrea gigas
0.000728
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
0.000827
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Crassostrea gigas
0.00477
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
0.00518
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S Mus musculus
0.00538
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
0.0065
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Acropora millepora
0.00858
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
0.00992
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S Mus musculus
0.0116
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
0.012
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/N152S Mus musculus
0.0132
-
L-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
0.0141
-
D-serine pH 8.0, 30°C, recombinant mutant H150S Mus musculus
0.0402
-
D-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.0447
-
L-serine pH 8.0, 30°C, recombinant wild-type enzyme Mus musculus
0.0478
-
D-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
0.0484
-
L-serine pH 8.0, 30°C, recombinant mutant N152S Mus musculus
0.0495
-
L-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
0.0537
-
D-serine pH 8.0, 30°C, recombinant mutant P151S/N152S Mus musculus
0.191
-
L-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus
0.195
-
D-serine pH 8.0, 30°C, recombinant mutant P151S Mus musculus
0.226
-
D-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon
0.268
-
L-serine pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S Penaeus monodon