Cloned (Comment) | Organism |
---|---|
gene SRR, sequence comparisons and phylogenetic tree | Mus musculus |
sequence comparisons and phylogenetic tree | Crassostrea gigas |
sequence comparisons and phylogenetic tree | Penaeus monodon |
sequence comparisons and phylogenetic tree | Acropora millepora |
Protein Variants | Comment | Organism |
---|---|---|
H150S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
H150S/N152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
H150S/P151S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
H150S/P151S/F152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Penaeus monodon |
H150S/P151S/N152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
additional information | introducing the triple serine loop region into SerRs promotes aspartate racemization | Mus musculus |
additional information | introducing the triple serine loop region into SerRs promotes aspartate racemization | Crassostrea gigas |
additional information | introducing the triple serine loop region into SerRs promotes aspartate racemization | Penaeus monodon |
additional information | introducing the triple serine loop region into SerRs promotes aspartate racemization | Acropora millepora |
N152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
P150S/P151S/F152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Acropora millepora |
P150S/P151S/Y152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Crassostrea gigas |
P151S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
P151S/F152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Acropora millepora |
P151S/N152S | site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk kinetics | Mus musculus | |
additional information | - |
additional information | Lineweaver-Burk kinetics | Crassostrea gigas | |
additional information | - |
additional information | Lineweaver-Burk kinetics | Penaeus monodon | |
additional information | - |
additional information | Lineweaver-Burk kinetics | Acropora millepora | |
7.71 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
8.48 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
12.7 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
14.9 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
15.1 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
18 | - |
L-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
19.2 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
20.9 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
21.4 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
21.5 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
28.9 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
28.9 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus | |
29.3 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
30 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus | |
37.4 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
38.1 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
42.4 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
42.6 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
44.1 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
45.1 | - |
D-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
50.6 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
53.4 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
55.2 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
69.7 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
69.9 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
119 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
125 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon | |
146 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
172 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
252 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Mus musculus | - |
D-serine | - |
r | |
L-serine | Crassostrea gigas | - |
D-serine | - |
r | |
L-serine | Penaeus monodon | - |
D-serine | - |
r | |
L-serine | Acropora millepora | - |
D-serine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acropora millepora | - |
- |
- |
Crassostrea gigas | A0A0U5AKI6 | - |
- |
Mus musculus | Q9QZX7 | - |
- |
Penaeus monodon | A0A0U4MRI4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Mus musculus | D-serine | - |
r | |
L-serine | - |
Crassostrea gigas | D-serine | - |
r | |
L-serine | - |
Penaeus monodon | D-serine | - |
r | |
L-serine | - |
Acropora millepora | D-serine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SerR | - |
Mus musculus |
SerR | - |
Crassostrea gigas |
SerR | - |
Penaeus monodon |
SerR | - |
Acropora millepora |
SRR | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
assay at | Penaeus monodon |
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Crassostrea gigas |
30 | - |
assay at | Acropora millepora |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00178 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
0.00211 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
0.00264 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
0.00366 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
0.00392 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
0.00423 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
0.00488 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
0.00772 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
0.00861 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
0.00871 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
0.0335 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon | |
0.0414 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
0.0551 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
0.057 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon | |
0.277 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
0.285 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.376 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
0.502 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
0.516 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
0.523 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
0.605 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
0.611 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
0.816 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
0.824 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
0.84 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.872 | - |
L-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
2.16 | - |
D-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
3.74 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
5.68 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus | |
5.71 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mus musculus |
8 | - |
assay at | Crassostrea gigas |
8 | - |
assay at | Penaeus monodon |
8 | - |
assay at | Acropora millepora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus | |
pyridoxal 5'-phosphate | - |
Crassostrea gigas | |
pyridoxal 5'-phosphate | - |
Penaeus monodon | |
pyridoxal 5'-phosphate | - |
Acropora millepora |
General Information | Comment | Organism |
---|---|---|
evolution | SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis | Mus musculus |
evolution | SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity | Crassostrea gigas |
evolution | SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity | Penaeus monodon |
evolution | SerRs and AspRs are not separated by their racemase functions and form a serine/aspartate racemase family cluster based on phylogenetic analysis, the organism has two paralogous genes, SerR and AspR. The presence of the triple serine loop region in both AspRs and SerRs leads to greater AspR activity while removing the triple serine loop region results in almost complete loss of AspR activity | Acropora millepora |
additional information | role of individual residues at position 150-152, overview | Mus musculus |
additional information | role of individual residues at position 150-152, overview | Crassostrea gigas |
additional information | role of individual residues at position 150-152, overview | Penaeus monodon |
additional information | role of individual residues at position 150-152, overview | Acropora millepora |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000559 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
0.0000651 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Penaeus monodon | |
0.000137 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
0.000138 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/F152S | Acropora millepora | |
0.000174 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
0.000205 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/F152S | Acropora millepora | |
0.000324 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
0.000338 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P150S/P151S/Y152S | Crassostrea gigas | |
0.000728 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
0.000827 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Crassostrea gigas | |
0.00477 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
0.00518 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/N152S | Mus musculus | |
0.00538 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
0.0065 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Acropora millepora | |
0.00858 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
0.00992 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S | Mus musculus | |
0.0116 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
0.012 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/N152S | Mus musculus | |
0.0132 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
0.0141 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S | Mus musculus | |
0.0402 | - |
D-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.0447 | - |
L-serine | pH 8.0, 30°C, recombinant wild-type enzyme | Mus musculus | |
0.0478 | - |
D-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
0.0484 | - |
L-serine | pH 8.0, 30°C, recombinant mutant N152S | Mus musculus | |
0.0495 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
0.0537 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S/N152S | Mus musculus | |
0.191 | - |
L-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus | |
0.195 | - |
D-serine | pH 8.0, 30°C, recombinant mutant P151S | Mus musculus | |
0.226 | - |
D-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon | |
0.268 | - |
L-serine | pH 8.0, 30°C, recombinant mutant H150S/P151S/F152S | Penaeus monodon |