Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-dithiothreitol | chemical reduction with DTT increases the enzyme activity by elevating Vmax | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystamine | abolishes the enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetic analysis | Homo sapiens | |
1 | 4 | L-serine | pH 8.0, 37°C, recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, recombinant His-tagged enzyme, SDS-PAGE, the major oligomeric form of recombinant enzyme in aqueous solution. The enzyme is most active as a noncovalent dimer containing one or more free sulfhydryls in the enzymes active center or a modulatory site | Homo sapiens |
87500 | - |
recombinant His-tagged enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | Homo sapiens | - |
D-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
central nervous system | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Homo sapiens | D-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, recombinant His-tagged enzyme, SDS-PAGE, the major oligomeric form of recombinant enzyme in aqueous solution. The enzyme is most active as a noncovalent dimer containing one or more free sulfhydryls in the enzyme's active center or a modulatory site | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | roles of quaternary structure and cysteine residues in the activity of human serine racemase, structure-function relationships of the recombinant enzyme, overview | Homo sapiens |
physiological function | D-serine, synthesized by the enzyme, is an important coagonist at the NR1 subunit of the NMDA receptor class of glutamate receptors | Homo sapiens |