Literature summary for 5.1.1.18 extracted from

Ito, T.; Murase, H.; Maekawa, M.; Goto, M.; Hayashi, S.; Saito, H.; Maki, M.; Hemmi, H.; Yoshimura, T.
Metal ion dependency of serine racemase from Dictyostelium discoideum (2012), Amino Acids, 43, 1567-1576.

Search for more literature for 5.1.1.18

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta II (DE3)	Dictyostelium discoideum

Protein Variants

Protein Variants	Comment	Organism
D213A	site-directed mutagenesis, the mutation abolishes the enzyme activation by Mg2+ and Na+	Dictyostelium discoideum
E207A	site-directed mutagenesis, the mutation abolishes the enzyme activation by Mg2+ and Na+	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no effect by EDTA	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics of wild-type and mutant enzymes for serine dehydration activity, EC 4.3.1.17, overview	Dictyostelium discoideum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates racemase racemization at 1 mM in Tris-HCl buffer, but inhibits racemase and dehydrase activities by 50% at 1 mM in borate-NaOH buffer	Dictyostelium discoideum
Mg2+	activates two- to ninefold racemization and dehydration at 1 mM in Tris-HCl buffer, but inhibits racemase and dehydrase activities by 50% at 1 mM in borate-NaOH buffer, half-maximal activation concentration is 0.0012 mM, Mg2+ and Na+ share the common metal ion-binding site	Dictyostelium discoideum
additional information	the enzyme is unique in its stimulation by monovalent cations such as Na+ in addition to Mg2+ and Ca2+	Dictyostelium discoideum
Na+	activates two- to ninefold racemization and dehydration, half-maximal activation concentration is 2.2 mM, Mg2+ and Na+ share the common metal ion-binding site	Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	2 * 35000, recombinant His-tagged enzyme, SDS-PAGE	Dictyostelium discoideum
74400	-	recombinant His-tagged enzyme, gel filtration	Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine	Dictyostelium discoideum	-	D-serine	-	?

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta II (DE3) by nickel affinity chromatography	Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine	-	Dictyostelium discoideum	D-serine	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, recombinant His-tagged enzyme, SDS-PAGE	Dictyostelium discoideum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	10.5	purified recombinant enzyme, stable at	Dictyostelium discoideum

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on	Dictyostelium discoideum

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Release 2023.1 (January 2023)