Literature summary for 5.1.1.18 extracted from

Gogami, Y.; Ito, K.; Kamitani, Y.; Matsushima, Y.; Oikawa, T.
Occurrence of D-serine in rice and characterization of rice serine racemase (2009), Phytochemistry, 70, 380-387.

Search for more literature for 5.1.1.18

Cloned(Commentary)

Cloned (Comment)	Organism
gene serR, encoding the enzyme, is located on chromosome 4 of the genomic DNA, phylogenetic analysis, cloning and expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)	Oryza sativa

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	ATP decreases the serine racemase activity of SerR but increases the serine dehydratase activity	Oryza sativa
additional information	SerR was inhibited by pyridoxal 5'-phosphate-enzyme inhibitors	Oryza sativa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Mg2+ increases the catalytic efficiency of the serine racemase activity of SerR and decreases that of the serine dehydratase activity. The structure of SerR is distorted by the addition of Mg2+, which is probably involved in the enzyme regulation	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
87000	-	recombinant enzyme, gel filtration	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine	Oryza sativa	-	D-serine	-	r

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q7XSN8	ssp. Japonica cv. Nipponbare or Koshihikari, gene serR	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged SerR 16.9fold from Escherichia coli strain BL21 (DE3) to homogeneity ny nickel affinity chromatography	Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.13	-	purified recombinant enzyme	Oryza sativa

Storage Stability

Storage Stability	Organism
4°C, purified recombinant enzyme, 20 mM sodium phosphate buffer, pH 8.0, 5 days, no loss of activity, but in 20 mM sodium or potassium phosphate buffer, pH 7.0, the enzyme is unstable and becomes inactivated, forming aggregates, leading to loss of about 30% of the initial activity after storage for 3 days	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine	-	Oryza sativa	D-serine	-	r
L-serine	SerR is highly specific for serine	Oryza sativa	D-serine	-	r
additional information	in addition to a serine racemase reaction, SerR catalyzes D- and L-serine dehydratase reactions, where the two compounds are converted to pyruvate, overview	Oryza sativa	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 x 34500, SDS-PAGE	Oryza sativa

Synonyms

Synonyms	Comment	Organism
SerR	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on	Oryza sativa

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Release 2023.1 (January 2023)