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Literature summary for 5.1.1.18 extracted from

  • Ohnishi, M.; Saito, M.; Wakabayashi, S.; Ishizuka, M.; Nishimura, K.; Nagata, Y.; Kasai, S.
    Purification and characterization of serine racemase from a hyperthermophylum Pyrobaculum islandicum (2007), J. Bacteriol., 190, 1359-1365.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.1.18

Cloned(Commentary)

Cloned (Comment) Organism
gene srr, DNA and amino acid sequence determination and anaylsis, expression in Escherichia coli Pyrobaculum islandicum

Inhibitors

Inhibitors Comment Organism Structure
ATP 10-20% inhibition; 10-20% inhibition at 0.1-1 mM Pyrobaculum islandicum
Cu2+ 30-40% inhibition Pyrobaculum islandicum
Fe2+ 20-30% inhibition; 20-30% inhibition Pyrobaculum islandicum
hydroxylamine complete inhibition at 1 mM Pyrobaculum islandicum
additional information no inhibition by p-chloromercuribenzoate, iodoacetamide, and iodoacetate Pyrobaculum islandicum
Sodium borohydride complete inhibition at 1 mM Pyrobaculum islandicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme shows no allosteric properties, it is not affected by either L-isoleucine or L-valine, or most of the metal ions Pyrobaculum islandicum
additional information
-
 additional information enzyme shows no allosteric properties Pyrobaculum islandicum
95.4
-
 D-serine pH 8.2, 95°C Pyrobaculum islandicum
185
-
 L-serine pH 8.2, 95°C Pyrobaculum islandicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Pyrobaculum islandicum
Cu2+ 30-40% stimulation Pyrobaculum islandicum
Mg2+ activates Pyrobaculum islandicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 4 * 44000, native enzyme, SDS-PAGE Pyrobaculum islandicum
44000
-
 3 * 44000, SDS-PAGE Pyrobaculum islandicum
130000
-
 gel filtration Pyrobaculum islandicum
137000
-
 native enzyme, gel filtration Pyrobaculum islandicum
143000
-
 native enzyme, light scattering-refraction Pyrobaculum islandicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Pyrobaculum islandicum
-
 D-serine
-
 r
L-threonine Pyrobaculum islandicum
-
 D-threonine
-
 r

Organism

Organism UniProt Comment Textmining
Pyrobaculum islandicum
-
 bifunctional enzyme, shows additional L-serine/L-threonine dehydratase activity
-
Pyrobaculum islandicum Q2V0H1 anaerobic hyperthermophilic archaeon, strain DSM 4184, gene srr
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography, recombinant enzyme from Escherichia coli by treatment with L-arginine and anion exchange chromatography Pyrobaculum islandicum

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimal growth temperature of the organism is 100°C Pyrobaculum islandicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.6
-
 pH 8.2, 95°C Pyrobaculum islandicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine 38% of the activity with L-serine Pyrobaculum islandicum L-serine
-
 r
D-threonine 13% of the activity with L-serine Pyrobaculum islandicum L-threonine
-
 r
L-serine
-
 Pyrobaculum islandicum D-serine
-
 r
L-serine the forward reaction is preferred, serine is preferred to threonine Pyrobaculum islandicum D-serine
-
 r
L-threonine
-
 Pyrobaculum islandicum D-threonine
-
 r
L-threonine the forward reaction is preferred, serine is preferred to threonine Pyrobaculum islandicum D-threonine
-
 r
L-threonine 40% of the activity with L-serine Pyrobaculum islandicum D-threonine
-
 r
additional information substrate specificity, the bifunctional enzyme shows high L-serine/L-threonine dehydratase activity, overview Pyrobaculum islandicum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 44000, native enzyme, SDS-PAGE Pyrobaculum islandicum
trimer 3 * 44000, SDS-PAGE Pyrobaculum islandicum

Synonyms

Synonyms Comment Organism
SRR
-
 Pyrobaculum islandicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Pyrobaculum islandicum
95
-
 both racemase and dehydratase activity Pyrobaculum islandicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5’-phosphate, 95.2% remaining activity Pyrobaculum islandicum
75
-
 8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5’-phosphate, 67.1% remaining activity Pyrobaculum islandicum
95
-
 8 h, purified enzyme, 100 mM Tris-HCl buffer, pH 7.8, containing 0.01 mM pyridoxal 5’-phosphate, 29.8% remaining activity Pyrobaculum islandicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.22
-
 D-serine pH 8.2, 95°C Pyrobaculum islandicum
14.8
-
 L-serine pH 8.2, 95°C Pyrobaculum islandicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Pyrobaculum islandicum
8.2
-
 racemase activity Pyrobaculum islandicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pyrobaculum islandicum
pyridoxal 5'-phosphate dependent on Pyrobaculum islandicum