Application | Comment | Organism |
---|---|---|
synthesis | epimerization is a rate-limiting step in cephalosporin biosynthesis in Acremonium chrysogenum. When epimerase activity is enhanced, there is a clear increase in cephalosporin production. Thus, this method provides a strategy that could easily be applied to improve industrial cephalosporin producing strains | Acremonium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopenicillin N | Acremonium chrysogenum | - |
penicillin N | - |
? | |
isopenicillin N | Acremonium chrysogenum C10 / ATCC 48272 | - |
penicillin N | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474 | - |
Acremonium chrysogenum C10 / ATCC 48272 | - |
and transformant strains TMCD2, TMCD26, TMCD32, TMCD39, TMCD53, TMCD242, TMCD474 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Acremonium chrysogenum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
strain C10 (ATCC 48272): 93 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD2: no activity, transformant strain TMCD26: 223 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD32: no activity, transformant strain TMCD39: no activity, transformant strain TMCD53: 226 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD242: 234 ng of cephalosporin C formed per min and mg of protein, transformant strain TMCD474: 202 ng of cephalosporin C formed per min and mg of protein | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopenicillin N | - |
Acremonium chrysogenum | penicillin N | - |
? | |
isopenicillin N | - |
Acremonium chrysogenum C10 / ATCC 48272 | penicillin N | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPN epimerase | - |
Acremonium chrysogenum |
isopenicillin N-CoA epimerase | - |
Acremonium chrysogenum |