Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 1 mM, 88% inhibition | Streptomyces clavuligerus | |
D-cycloserine | 1 mM, 23% inhibition | Streptomyces clavuligerus | |
FAD | 0.5 mM, 80% inhibition | Streptomyces clavuligerus | |
fluorescamine | inactivation at molar ratios higher than 2 | Streptomyces clavuligerus | |
hydroxylamine | 1 mM, 95% inhibition | Streptomyces clavuligerus | |
iodoacetamide | 1 mM, 28% inhibition | Streptomyces clavuligerus | |
Iproniazid | 1 mM, 25% inhibition | Streptomyces clavuligerus | |
isoniazid | 1 mM, 23% inhibition | Streptomyces clavuligerus | |
N-ethylmaleimide | 1 mM, 45% inhibition | Streptomyces clavuligerus | |
Phenylglyoxal | 1 mM, 33% inhibition | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
pyridoxal 5'-phosphate | - |
Streptomyces clavuligerus | |
0.3 | - |
isopenicillin N | - |
Streptomyces clavuligerus | |
0.78 | - |
Penicillin N | - |
Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
1 * 47000, SDS-PAGE | Streptomyces clavuligerus |
50000 | - |
gel filtration | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopenicillin N | Streptomyces clavuligerus | - |
penicillin N | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DE-52, DEAE-Affi-gel blue, Sephadex G-200, calcium phosphate, Mono Q | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.85 | - |
isopenicillin N epimerization | Streptomyces clavuligerus |
7.77 | - |
penicillin N epimerization | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deacetoxycephalosporin C | less than 1% of activity with penicillin N | Streptomyces clavuligerus | ? | - |
? | |
isopenicillin | - |
Streptomyces clavuligerus | penicillin N | - |
r | |
isopenicillin N | - |
Streptomyces clavuligerus | penicillin N | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Streptomyces clavuligerus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
rapid denaturation above | Streptomyces clavuligerus |
60 | - |
complete inactivation after 10 min | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.3 | - |
Streptomyces clavuligerus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.3 | 10 | stable at 4°C for 24 h | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1 mol of pyridoxal 5'-phosphate per mol enzyme, 1.5fold increase in activity in the presence of 0.01 mM pyridoxal 5'-phosphate | Streptomyces clavuligerus |