Application | Comment | Organism |
---|---|---|
synthesis | the enzyme might be useful for applications in dynamic kinetic resolution for D- or L-amino acid production | Achromobacter obae |
synthesis | the enzyme might be useful for applications in dynamic kinetic resolution for D- or L-amino acid production | Citreicella sp. SE45 |
Cloned (Comment) | Organism |
---|---|
gene CSE45_2055, sequence comparisons, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Achromobacter obae |
gene CSE45_2055, sequence comparisons, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Citreicella sp. SE45 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 44% inhibition at 5 mM | Citreicella sp. SE45 | |
Co2+ | 59% inhibition at 5 mM | Citreicella sp. SE45 | |
Cu2+ | 92% inhibition at 5 mM | Citreicella sp. SE45 | |
Fe2+ | 97% inhibition at 5 mM | Citreicella sp. SE45 | |
Mg2+ | 45% inhibition at 5 mM | Citreicella sp. SE45 | |
Mn2+ | 49% inhibition at 5 mM | Citreicella sp. SE45 | |
additional information | poor inhibition by EDTA at 2-10 mM | Citreicella sp. SE45 | |
Ni2+ | 55% inhibition at 5 mM | Citreicella sp. SE45 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.17 | - |
L-2-aminohexano-6-lactam | recombinant enzyme, pH 7.5, 30°C | Citreicella sp. SE45 | |
5.3 | - |
D-2-aminohexano-6-lactam | recombinant enzyme, pH 7.5, 30°C | Citreicella sp. SE45 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-aminohexano-6-lactam | Achromobacter obae | - |
L-2-aminohexano-6-lactam | - |
r | |
L-2-aminohexano-6-lactam | Achromobacter obae | - |
D-2-aminohexano-6-lactam | - |
r | |
additional information | Citreicella sp. SE45 | the enzyme encoded by gene CSE45_2055 is a 2,2-dialkylglycine decarboxylase, EC 4.1.1.64 (UniProt ID D0D2T1), that shows enhanced alpha-amino-epsilon-caprolactam racemase activity on D- and L-2-aminohexano-6-lactams | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter obae | Q7M181 | - |
- |
Citreicella sp. SE45 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 129fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Citreicella sp. SE45 |
recombinant His-tagged enzyme 27.9fold from Escherichia coli strain BL21(DE3) by heat treatment, nickel affinity chromatography, and dialysis | Achromobacter obae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
purified recombinant enzyme, substrate L-valine amide, pH 7.5, 30°C | Citreicella sp. SE45 |
0.06 | - |
purified recombinant enzyme, substrate L-valine amide, pH 7.5, 30°C | Achromobacter obae |
0.1 | - |
purified recombinant enzyme, substrate L-phenylglycine, pH 7.5, 30°C | Achromobacter obae |
0.1 | - |
purified recombinant enzyme, substrate L-phenylglycine, pH 7.5, 30°C | Citreicella sp. SE45 |
0.2 | - |
purified recombinant enzyme, substrate L-phenylalanine, pH 7.5, 30°C | Achromobacter obae |
0.3 | - |
purified recombinant enzyme, substrate L-phenylalanine, pH 7.5, 30°C | Citreicella sp. SE45 |
0.8 | - |
purified recombinant enzyme, substrate L-methionine amide, pH 7.5, 30°C | Achromobacter obae |
0.9 | - |
purified recombinant enzyme, substrate L-methionine amide, pH 7.5, 30°C | Citreicella sp. SE45 |
1.5 | - |
purified recombinant enzyme, substrate L-leucine amide, pH 7.5, 30°C | Citreicella sp. SE45 |
1.8 | - |
purified recombinant enzyme, substrate L-leucine amide, pH 7.5, 30°C | Achromobacter obae |
5.3 | - |
crude recombinant enzyme, substrate L-2-aminohexano-6-lactam, pH 7.5, 30°C | Citreicella sp. SE45 |
16 | - |
purified recombinant enzyme, substrate L-serine amide, pH 7.5, 30°C | Achromobacter obae |
16.7 | - |
purified recombinant enzyme, substrate L-alanine amide, pH 7.5, 30°C | Achromobacter obae |
17.5 | - |
purified recombinant enzyme, substrate L-alanine amide, pH 7.5, 30°C | Citreicella sp. SE45 |
21.6 | - |
purified recombinant enzyme, substrate L-serine amide, pH 7.5, 30°C | Citreicella sp. SE45 |
443 | - |
purified recombinant enzyme, substrate L-aspartate, pH 7.5, 30°C | Achromobacter obae |
627 | - |
purified recombinant enzyme, substrate D-aspartate, pH 7.5, 30°C | Achromobacter obae |
681 | - |
purified recombinant enzyme, substrate L-2-aminohexano-6-lactam, pH 7.5, 30°C | Citreicella sp. SE45 |
772 | - |
purified recombinant enzyme, substrate D-2-aminohexano-6-lactam, pH 7.5, 30°C | Citreicella sp. SE45 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-aminohexano-6-lactam | - |
Achromobacter obae | L-2-aminohexano-6-lactam | - |
r | |
D-2-aminohexano-6-lactam | preferred substrate | Achromobacter obae | L-2-aminohexano-6-lactam | - |
r | |
D-2-aminohexano-6-lactam | preferred substrate | Citreicella sp. SE45 | L-2-aminohexano-6-lactam | - |
r | |
L-2-aminohexano-6-lactam | - |
Achromobacter obae | D-2-aminohexano-6-lactam | - |
r | |
L-2-aminohexano-6-lactam | high activity | Achromobacter obae | D-2-aminohexano-6-lactam | - |
r | |
L-2-aminohexano-6-lactam | high activity | Citreicella sp. SE45 | D-2-aminohexano-6-lactam | - |
r | |
L-alanine amide | - |
Achromobacter obae | D-alanine amide | - |
r | |
L-alanine amide | - |
Citreicella sp. SE45 | D-alanine amide | - |
r | |
L-leucine amide | - |
Achromobacter obae | D-leucine amide | - |
r | |
L-leucine amide | - |
Citreicella sp. SE45 | D-leucine amide | - |
r | |
L-methionine amide | - |
Achromobacter obae | D-methionine amide | - |
r | |
L-methionine amide | - |
Citreicella sp. SE45 | D-methionine amide | - |
r | |
L-phenylalanine amide | - |
Achromobacter obae | D-phenylalanine amide | - |
r | |
L-phenylalanine amide | - |
Citreicella sp. SE45 | D-phenylalanine amide | - |
r | |
L-phenylglycine amide | - |
Achromobacter obae | D-phenylglycine amide | - |
r | |
L-phenylglycine amide | - |
Citreicella sp. SE45 | D-phenylglycine amide | - |
r | |
L-serine amide | - |
Achromobacter obae | D-serine amide | - |
r | |
L-serine amide | - |
Citreicella sp. SE45 | D-serine amide | - |
r | |
L-valine amide | - |
Achromobacter obae | D-valine amide | - |
r | |
L-valine amide | - |
Citreicella sp. SE45 | D-valine amide | - |
r | |
additional information | the enzyme encoded by gene CSE45_2055 is a 2,2-dialkylglycine decarboxylase, EC 4.1.1.64 (UniProt ID D0D2T1), that shows enhanced alpha-amino-epsilon-caprolactam racemase activity on D- and L-2-aminohexano-6-lactams | Citreicella sp. SE45 | ? | - |
? | |
additional information | The enzyme acts on a broad range of amino acid amides, particularly unbranched amino acid amides including L-alanine amide and L-serine amide. No activity with L-tyrosine amide | Achromobacter obae | ? | - |
? | |
additional information | The enzyme acts on a broad range of amino acid amides, particularly unbranched amino acid amides including L-alanine amide and L-serine amide. No activity with L-tyrosine amide | Citreicella sp. SE45 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Achromobacter obae |
? | x * 47400, recombinant His-tagged enzyme, SDS-PAGE | Citreicella sp. SE45 |
Synonyms | Comment | Organism |
---|---|---|
ACL racemase | - |
Achromobacter obae |
ACL racemase | - |
Citreicella sp. SE45 |
ACLR | - |
Achromobacter obae |
ACLR | - |
Citreicella sp. SE45 |
alpha-Amino-epsilon-caprolactam racemase | - |
Achromobacter obae |
alpha-Amino-epsilon-caprolactam racemase | - |
Citreicella sp. SE45 |
More | cf. EC 5.1.1.10 | Achromobacter obae |
More | cf. EC 5.1.1.10 | Citreicella sp. SE45 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Achromobacter obae |
70 | - |
- |
Citreicella sp. SE45 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | recombinant enzyme, activity range, enzyme activity rapidly increases between 40°C and 60°C and reaches its maximum at 70°C, above 70°C, the enzyme activity declines | Citreicella sp. SE45 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant His-tagged enzyme, the enzyme retains more than 90% of its activity after being incubated at 50°C for 50 min, and enzyme activity significantly decreases at temperatures greater than 50°C | Citreicella sp. SE45 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
465 | - |
L-2-aminohexano-6-lactam | recombinant enzyme, pH 7.5, 30°C | Citreicella sp. SE45 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Achromobacter obae |
10 | - |
- |
Citreicella sp. SE45 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 12 | active in a broad pH range, with more than 80 % activity in the pH range of pH 7.5-11.0, maximal activity at pH 10.0 | Citreicella sp. SE45 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | purified recombinant His-tagged enzyme, highly stable after 1 h incubation at 4°C, not stable under acidic conditions | Citreicella sp. SE45 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Citreicella sp. SE45 | |
pyridoxal 5'-phosphate | PLP, the enzyme is a fold-type I PLP-dependent enzyme | Achromobacter obae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
214 | - |
L-2-aminohexano-6-lactam | recombinant enzyme, pH 7.5, 30°C | Citreicella sp. SE45 |