Cloned (Comment) | Organism |
---|---|
gene Got1l1, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21 and in HEK-293T cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of Got1l1 knockout mice. Got1l1 mRNA is highly expressed in the testis in wild-type mice, but is not detected in Got1l1 knockout mice. The D-aspartate contents of knockout and Got1l1 knockout mice are not significantly different in the testis and hippocampus | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Mus musculus | - |
D-aspartate | - |
r | |
additional information | Mus musculus | recombinant Got1l1 protein is a pyridoxal 5'-phosphate-dependent enzyme and synthesizes substantial D-aspartate from L-aspartate and only one-fifth as much L-glutamate, with very little D-glutamate in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q7TSV6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21 and HEK-293T cells by nickel affinity chromatography and desalting gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no expression of Got1l1 mRNA in the brain and submandibular gland, where D-aspartate is abundant | Mus musculus | - |
testis | Got1l1 mRNA is highly expressed in the testis | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Mus musculus | D-aspartate | - |
r | |
additional information | recombinant Got1l1 protein is a pyridoxal 5'-phosphate-dependent enzyme and synthesizes substantial D-aspartate from L-aspartate and only one-fifth as much L-glutamate, with very little D-glutamate in vitro | Mus musculus | ? | - |
? | |
additional information | recombinant Got1l1 expressed in mammalian cells shows L-aspartate aminotransferase activity, but lacks aspartate racemase activity. When L-aspartate and 2-oxoglutarate are reacted with the Got1l1 purified from HEK293T cells, neither D-aspartate nor D-glutamate is generated, but a detectable amount of L-glutamate is obtained | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GOT1L1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | D-aspartate contents of wild-type and Got1l1 knockout mice are not significantly different in the testis and hippocampus. The recombinant Got1l1 expressed in mammalian cells shows L-aspartate aminotransferase activity, but lacks aspartate racemase activity | Mus musculus |
metabolism | enzyme Got1l1 is not the major aspartate racemase, there might be another D-aspartate-synthesizing enzyme | Mus musculus |
physiological function | in the hippocampus, D-aspartate strongly enhances N-methyl-D-aspartate receptor-dependent long-term potentiation and is involved in learning and memory | Mus musculus |