Literature summary for 5.1.1.13 extracted from

Fujii, T.; Yamauchi, T.; Ishiyama, M.; Gogami, Y.; Oikawa, T.; Hata, Y.
Crystallographic studies of aspartate racemase from Lactobacillus sakei NBRC 15893 (2015), Acta Crystallogr. Sect. F, 71, 1012-1016 .

Application

Application	Comment	Organism
food industry	the enzyme from the lactic acid bacterium Lactobacillus sakei NBRC 15893 is considered to be involved in D-aspartate synthesis during the brewing process of Japanese sake at low temperatures	Latilactobacillus sakei

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)	Latilactobacillus sakei

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, sitting drop vapour diffusion method with microseeding, mixing of 0.002 ml of 20 mg/ml protein in 50 mM MES, pH 6.5, and 4 mM dithiothreitol, with 0.002 ml of reservoir solution containing 25% v/v PEG MME 550, and 5% v/v 2-propanol, and 0.1 M sodium acetate, pH 4.8, equilibration againat 0.1 ml reservoir solution, 20°C, single rod-shaped crystals, X-ray diffraction structure determination and analysis at 2.55 A resolution, molecular replacement calculations are carried out using the coordinates of PhAspR, PDB ID 1jfl, as a starting model	Latilactobacillus sakei

Crystallization (Comment)

Organism

purified enzyme, sitting drop vapour diffusion method with microseeding, mixing of 0.002 ml of 20 mg/ml protein in 50 mM MES, pH 6.5, and 4 mM dithiothreitol, with 0.002 ml of reservoir solution containing 25% v/v PEG MME 550, and 5% v/v 2-propanol, and 0.1 M sodium acetate, pH 4.8, equilibration againat 0.1 ml reservoir solution, 20°C, single rod-shaped crystals, X-ray diffraction structure determination and analysis at 2.55 A resolution, molecular replacement calculations are carried out using the coordinates of PhAspR, PDB ID 1jfl, as a starting model

Latilactobacillus sakei

Organism

Organism	UniProt	Comment	Textmining
Latilactobacillus sakei	-	isolated from kimoto	-
Latilactobacillus sakei NBRC 15893	-	isolated from kimoto	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration	Latilactobacillus sakei

Synonyms

Synonyms	Comment	Organism
LsAspR	-	Latilactobacillus sakei

General Information

General Information	Comment	Organism
physiological function	the enzyme from the lactic acid bacterium Lactobacillus sakei NBRC 15893 is considered to be involved in D-aspartate synthesis during the brewing process of Japanese sake at low temperatures	Latilactobacillus sakei