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Literature summary for 5.1.1.10 extracted from

  • Würges, K.; Petrusevska, K.; Serci, S.; Wilhelm, S.; Wandrey, C.; Seidel-Morgenstern, A.; Elsner, M.; Lütz, S.
    Enzyme-assisted physicochemical enantioseparation processes: Part I. Production and characterization of a recombinant amino acid racemase (2009), J. Mol. Catal. B, 58, 10-16.
No PubMed abstract available
Search for more literature for 5.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
over-expressed in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 L-Met crude lyophilisates, 25°C, pH 7, Vmax: 2150 micromol/min/g Pseudomonas putida
2 3 D-Met crude lyophilisates, 30°C, pH 7, Vmax: 2075 micromol/min/g Pseudomonas putida
18
-
 D-Asn pure lyophilisates, 20°C, pH 7, Vmax: 770 micromol/min/g Pseudomonas putida
20
-
 L-Met crude lyophilisates, 25°C, pH 8, Vmax: 2951 micromol/min/g Pseudomonas putida
22
-
 D-Met crude lyophilisates, 25°C, pH 7, Vmax: 1892 micromol/min/g Pseudomonas putida
25
-
 D-Met crude lyophilisates, 20°C, pH 7, Vmax: 1696 micromol/min/g Pseudomonas putida
28
-
 D-Met crude lyophilisates, 25°C, pH 8, Vmax: 3627 micromol/min/g Pseudomonas putida
30
-
 L-Met crude lyophilisates, 25°C, pH 6, Vmax: 1174 micromol/min/g Pseudomonas putida
30
-
 L-Met crude lyophilisates, 30°C, pH 7, Vmax: 2296 micromol/min/g Pseudomonas putida
30
-
 D-Met crude lyophilisates, 35°C, pH 7, Vmax: 2512 micromol/min/g Pseudomonas putida
30
-
 L-Asn pure lyophilisates, 20°C, pH 7, Vmax: 1626 micromol/min/g Pseudomonas putida
31
-
 L-Met crude lyophilisates, 20°C, pH 7, Vmax: 1889 micromol/min/g Pseudomonas putida
32
-
 D-Met crude lyophilisates, 40°C, pH 7, Vmax: 2668 micromol/min/g Pseudomonas putida
35
-
 L-Met crude lyophilisates, 40°C, pH 7, Vmax: 2847 micromol/min/g Pseudomonas putida
39
-
 L-Met crude lyophilisates, 35°C, pH 7, Vmax: 2833 micromol/min/g Pseudomonas putida
42
-
 D-Met crude lyophilisates, 25°C, pH 6, Vmax: 1160 micromol/min/g Pseudomonas putida
47
-
 L-Asn pure lyophilisates, 25°C, pH 7, Vmax: 2427 micromol/min/g Pseudomonas putida
50
-
 D-Asn pure lyophilisates, 25°C, pH 7, Vmax: 1299 micromol/min/g Pseudomonas putida
62
-
 D-Asn pure lyophilisates, 30°C, pH 7, Vmax: 1881 micromol/min/g Pseudomonas putida
66
-
 D-Asn pure lyophilisates, 35°C, pH 7, Vmax: 2585 micromol/min/g Pseudomonas putida
76
-
 L-Asn pure lyophilisates, 30°C, pH 7, Vmax: 3930 micromol/min/g Pseudomonas putida
101
-
 L-Asn pure lyophilisates, 35°C, pH 7, Vmax: 5683 micromol/min/g Pseudomonas putida
103
-
 D-Asn pure lyophilisates, 40°C, pH 7, Vmax: 4032 micromol/min/g Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two enzyme lyophilisates of different purity are obtained from which the crude is sufficient for the racemization of methionine and the pure is used for asparagine Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Asn
-
 Pseudomonas putida L-Asn
-
 r
D-Met
-
 Pseudomonas putida L-Met
-
 r
L-Asn
-
 Pseudomonas putida D-Asn
-
 r
L-Met
-
 Pseudomonas putida D-Met
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 45000 Da, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
amino acid racemase
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas putida