Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas taetrolens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas taetrolens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
calculated from cDNA | Pseudomonas taetrolens |
84000 | - |
gel filtration | Pseudomonas taetrolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas taetrolens | I0J1I6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | N-terminal signal sequence is cleaved off | Pseudomonas taetrolens |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Pseudomonas taetrolens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1120 | - |
recombinant protein | Pseudomonas taetrolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala | 12% activity, compared to D-Lys | Pseudomonas taetrolens | L-Ala | - |
? | |
D-Arg | 95% activity, compared to D-Lys | Pseudomonas taetrolens | L-Arg | - |
? | |
D-ethionine | 11% activity, compared to D-Lys | Pseudomonas taetrolens | L-ethionine | - |
? | |
D-homoarginine | 6.2% activity, compared to D-Lys | Pseudomonas taetrolens | L-homoarginine | - |
? | |
D-Lys | 100% activity | Pseudomonas taetrolens | L-Lys | - |
? | |
D-Met | 12% activity, compared to D-Lys | Pseudomonas taetrolens | L-Met | - |
? | |
D-norvaline | 6.5% activity, compared to D-Lys | Pseudomonas taetrolens | L-norvaline | - |
? | |
D-ornithine | 42% activity, compared to D-Lys | Pseudomonas taetrolens | L-ornithine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41500 Da | Pseudomonas taetrolens |
Synonyms | Comment | Organism |
---|---|---|
amino acid racemase | - |
Pseudomonas taetrolens |
ArgR | - |
Pseudomonas taetrolens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas taetrolens |
65 | - |
maximum activity | Pseudomonas taetrolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
optimal activity | Pseudomonas taetrolens |
10 | - |
assay at | Pseudomonas taetrolens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas taetrolens |