Cloned (Comment) | Organism |
---|---|
amino acid racemase of Pseudomonas putida DSM 3263 is overexpressed in Escherichia coli and delivered cell free extract with easily sufficient activity (2050 U/mg total protein) for application in an enzyme membrane reactor (EMR) setting | Pseudomonas putida |
General Stability | Organism |
---|---|
buffer without organic content provides excellent stability at moderate temperatures (2035°C) while addition of 20% acetonitrile or methanol drastically reduces the half-life of the racemase | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | model-based calculations suggested that a methanol content of 10% and an acetonitrile content of 20% provide maximum productivity in enzyme membrane reactor (EMR) operations. However product concentrations are decreased in comparison to purely aqueous operation due to decreasing solubility of methionine with increasing organic solvent content. | Pseudomonas putida | |
4.1 | - |
L-methionine | 100% aqueous | Pseudomonas putida | |
4.8 | - |
D-methionine | 100% aqueous | Pseudomonas putida | |
7.8 | - |
L-methionine | 10% methanol | Pseudomonas putida | |
9 | - |
D-methionine | 10% methanol | Pseudomonas putida | |
9.4 | - |
L-methionine | 20% methanol | Pseudomonas putida | |
11.1 | - |
D-methionine | 10% acetonitril | Pseudomonas putida | |
11.5 | - |
L-methionine | 10% acetonitril | Pseudomonas putida | |
11.9 | - |
D-methionine | 40% acetonitril | Pseudomonas putida | |
13.2 | - |
L-methionine | 20% acetonitril | Pseudomonas putida | |
13.2 | - |
D-methionine | 20% acetonitril | Pseudomonas putida | |
15.8 | - |
D-methionine | 20% methanol | Pseudomonas putida | |
16.1 | - |
L-methionine | 40% acetonitril | Pseudomonas putida | |
22.3 | - |
L-methionine | 40% methanol | Pseudomonas putida | |
24.3 | - |
D-methionine | 40% methanol | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
DSM 3263 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Methionine | - |
Pseudomonas putida | D-Methionine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
amino-acid racemase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas putida |