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Literature summary for 5.1.1.10 extracted from
- Amino acid racemase with broad substrate specificity, its properties and use in phenylalanine racemization (1988), Appl. Microbiol. Biotechnol., 29, 523-527.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-cycloserine | - |
Pseudomonas putida |  |
| D-penicillamine | - |
Pseudomonas putida | |
| hydroxylamine | - |
Pseudomonas putida | |
| NiCl2 | - |
Pseudomonas putida | |
| ZnCl2 | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.8 | - |
D-Gln | - |
Pseudomonas putida | |
| 9.5 | - |
D-Met | - |
Pseudomonas putida | |
| 18 | - |
D-Ser | - |
Pseudomonas putida | |
| 25.7 | - |
D-Phe | - |
Pseudomonas putida | |
| 26 | - |
D-Phe | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Pseudomonas putida |
| 62000 | 65000 | gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 651 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arg | 21% of the activity relative to D-Gln | Pseudomonas putida | L-Arg | - |
? | |
| D-Asp | 6% of the activity relative to D-Gln | Pseudomonas putida | L-Asp | - |
? | |
| D-Gln | D-Glu is the best substrate | Pseudomonas putida | L-Gln | - |
? | |
| D-Phe | 1% of the velocity relative to D-Glu | Pseudomonas putida | L-Phe | - |
? | |
| L-Ala | D-Ala, 15% of the activity relative to D-Gln | Pseudomonas putida | D-Ala | - |
? | |
| L-Glu | no activity with D-Glu | Pseudomonas putida | D-Glu | - |
? | |
| L-Leu | D-Leu, 13% of the activity relative to D-Gln | Pseudomonas putida | D-Leu | - |
? | |
| L-Lys | D-Lys at 22% of the activity relative to D-Gln | Pseudomonas putida | D-Lys | - |
? | |
| L-Met | D-Met, 42% of the activity relative to D-Gln | Pseudomonas putida | D-Met | - |
? | |
| L-Ser | D-Ser, 31% of the activity relative to D-Gln | Pseudomonas putida | D-Ser | - |
? | |
| L-Thr | no activity | Pseudomonas putida | L-Thr | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 10.0, 10 min, stable | Pseudomonas putida |
| 80 | - |
pH 10.0, 10 min, most of the activity is lost | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | at 30°C, 1 h, stable | Pseudomonas putida |
| 10 | - |
at 50°C, 10 min, stable | Pseudomonas putida |
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