Cloned (Comment) | Organism |
---|---|
gene alr, recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
gene alr, recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-Chloro-D-alanine | BCDA, its primary target is glutamate racemase, poor activity oagainst alanine racemase activity, potent antituberculosis activity. BCDA does not inhibit the D-alanine pathway in intact cells, consistent with its poor in vitro activity, it is instead an irreversible mechanism-based inactivator of glutamate racemase (MurI), an upstream enzyme in the same early stage of peptidoglycan biosynthesis. Inhibition kinetics, overview. Glutamate racemase (MurI) is a pyridoxal 5'-phosphate-independent racemase and is therefore unable to undergo the same mechanism of inhibition as Alr with BCDA | Bacillus subtilis | |
beta-Chloro-D-alanine | BCDA, the compund is a very poor inhibitor of recombinant Mycobacterium tuberculosis Alr, despite having potent antituberculosis activity, its primary target is glutamate racemase, poor activity oagainst alanine racemase activity, potent antituberculosis activity. BCDA does not inhibit the D-alanine pathway in intact cells, consistent with its poor in vitro activity, it is instead an irreversible mechanism-based inactivator of glutamate racemase (MurI), an upstream enzyme in the same early stage of peptidoglycan biosynthesis. Inhibition kinetics, overview. Glutamate racemase (MurI) is a pyridoxal 5'-phosphate-independent racemase and is therefore unable to undergo the same mechanism of inhibition as Alr with BCDA | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
L-alanine | pH 7.6, 37°C | Mycobacterium tuberculosis | |
5.9 | - |
L-alanine | pH 7.6, 37°C | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | Bacillus subtilis | - |
D-alanine | - |
r | |
L-alanine | Mycobacterium tuberculosis | - |
D-alanine | - |
r | |
L-alanine | Bacillus subtilis 168 | - |
D-alanine | - |
r | |
L-alanine | Mycobacterium tuberculosis ATCC 25618 / H37Rv | - |
D-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P94556 | - |
- |
Bacillus subtilis 168 | P94556 | - |
- |
Mycobacterium tuberculosis | P9WPW9 | - |
- |
Mycobacterium tuberculosis ATCC 25618 / H37Rv | P9WPW9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage | Bacillus subtilis |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine | - |
Bacillus subtilis | D-alanine | - |
r | |
L-alanine | - |
Mycobacterium tuberculosis | D-alanine | - |
r | |
L-alanine | - |
Bacillus subtilis 168 | D-alanine | - |
r | |
L-alanine | - |
Mycobacterium tuberculosis ATCC 25618 / H37Rv | D-alanine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
MurI | - |
Bacillus subtilis |
MurI | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
L-alanine | pH 7.6, 37°C | Mycobacterium tuberculosis | |
1190 | - |
L-alanine | pH 7.6, 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |
7.6 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
beta-Chloro-D-alanine | pH 7.6, 37°C | Mycobacterium tuberculosis | |
0.23 | - |
beta-Chloro-D-alanine | pH 7.6, 37°C | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-alanine | pH 7.6, 37°C | Mycobacterium tuberculosis | |
201.7 | - |
L-alanine | pH 7.6, 37°C | Bacillus subtilis |