Literature summary for 5.1.1.1 extracted from

Palani, K.; Burley, S.K.; Swaminathan, S.
Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate (2013), Acta Crystallogr. Sect. F, 69, 15-19.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally His6-tagged selenomethionine-derivatized enzyme in Escherichia coli strain BL21(DE3) in HY medium	Oenococcus oeni

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminally His6-tagged selenomethionine-derivatized enzyme by sitting drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein solution with 0.002 ml of reservoir solution containing 25% PEG 3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M sodium chloride, and equilibration against 0.650 ml of reservoir solution, X-ray diffraction structure determination and analysis at 1.7 A resolution via single wavelength anomalous dispersion	Oenococcus oeni

Crystallization (Comment)

Organism

purified recombinant C-terminally His6-tagged selenomethionine-derivatized enzyme by sitting drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein solution with 0.002 ml of reservoir solution containing 25% PEG 3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M sodium chloride, and equilibration against 0.650 ml of reservoir solution, X-ray diffraction structure determination and analysis at 1.7 A resolution via single wavelength anomalous dispersion

Oenococcus oeni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alanine	Oenococcus oeni	catalyzes the interconversion of D-alanine and L-alanine	D-alanine	-	r
L-alanine	Oenococcus oeni PSU-1	catalyzes the interconversion of D-alanine and L-alanine	D-alanine	-	r

Organism

Organism	UniProt	Comment	Textmining
Oenococcus oeni	Q04DI1	locus OEOE_1641, strain ATCC BAA-331/PSU-1	-
Oenococcus oeni PSU-1	Q04DI1	locus OEOE_1641, strain ATCC BAA-331/PSU-1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged selenomethionine-derivatized enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Oenococcus oeni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine	catalyzes the interconversion of D-alanine and L-alanine	Oenococcus oeni	D-alanine	-	r
L-alanine	catalyzes the interconversion of D-alanine and L-alanine	Oenococcus oeni PSU-1	D-alanine	-	r

Subunits

Subunits	Comment	Organism
dimer	the enzyme exists as a symmetric dimer in the crystal, with both protomers contributing to the two active sites, a symmetric head-to-tail homodimer	Oenococcus oeni
More	enzyme sequence and structure comparisons, overview	Oenococcus oeni

Synonyms

Synonyms	Comment	Organism
ALR	-	Oenococcus oeni
OEOE_1641	locus name	Oenococcus oeni

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	dependent on, pyridoxal 5'-phosphate is bound to each monomer of the dimeric enzyme and forms a Schiff base with Lys39	Oenococcus oeni

General Information

General Information	Comment	Organism
evolution	alanine racemase belongs to the fold-type III group of pyridoxal 5'-phosphate-dependent enzymes	Oenococcus oeni