Literature summary for 5.1.1.1 extracted from

Saito, M.; Nishimura, K.; Hasegawa, Y.; Shinohara, T.; Wakabayashi, S.; Kurihara, T.; Ishizuka, M.; Nagata, Y.
Alanine racemase from Helicobacter pylori NCTC 11637: purification, characterization and gene cloning (2007), Life Sci., 80, 788-794.

Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.2	-	D-alanine	Vmax 37.9 micromol/min/mg	Helicobacter pylori
100	-	L-alanine	Vmax 909 micromol/min/mg	Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000, SDS-PAGE	Helicobacter pylori
68000	-	gel-filtration analysis	Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-alanine	Helicobacter pylori	-	D-alanine	-	r

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	Q1XG01	NCTC 11637	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
61.8	-	in presence of 50 mM L-alanine with a 5.0% yield	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine	-	Helicobacter pylori	D-alanine	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 37000, SDS-PAGE	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
alanine racemase	-	Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
37.9	-	D-alanine	-	Helicobacter pylori
632	-	L-alanine	-	Helicobacter pylori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	-	Helicobacter pylori

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Helicobacter pylori

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Release 2023.1 (January 2023)