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Literature summary for 5.1.1.1 extracted from

  • Kim, S.S.; Yu, Y.G.
    Molecular cloning of an extremely thermostable alanine racemase from Aquifex pyrophilus and enzymatic characterization of the expressed protein (2000), J. Biochem. Mol. Biol., 33, 82-88.
No PubMed abstract available
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a His-tagged protein Aquifex pyrophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 D-Ala 90°C, pH 9.5 Aquifex pyrophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Aquifex pyrophilus

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 50%, enzyme maintains more than 90% of its activity Aquifex pyrophilus
guanidine-HCl 4 M, maintains more than 60% of its activity Aquifex pyrophilus
isopropanol 50%, enzyme maintains more than 30% of its activity Aquifex pyrophilus
urea 2 M, maintains more than 60% of its activity Aquifex pyrophilus

Organism

Organism UniProt Comment Textmining
Aquifex pyrophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquifex pyrophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyric acid 18% of the activity with L-Ala Aquifex pyrophilus D-2-aminobutyric acid
-
 ?
L-Ala
-
 Aquifex pyrophilus D-Ala
-
 ?
L-Ser 50% of the activity with L-Ala Aquifex pyrophilus D-Ser
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Aquifex pyrophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aquifex pyrophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 100 35°C: about 75% of maximal activity, 100°C: about 55% of maximal activity Aquifex pyrophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 1 h, no denaturation Aquifex pyrophilus
95
-
 pH 8.0, 50 mM Tris-HCl, 0.5 M sodium phosphate, maintains more than 90% of its activity after 2 h Aquifex pyrophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.8
-
 D-Ala 90°C, pH 9.5 Aquifex pyrophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Aquifex pyrophilus

pH Range

pH Minimum pH Maximum Comment Organism
7 11 pH 7.0: about 75% of maximal activity, pH 11.0: about 75% of maximal activity Aquifex pyrophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 mol per mol of enzyme Aquifex pyrophilus