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Literature summary for 5.1.1.1 extracted from

  • Uo, T.; Yoshimura, T.; Tanaka, N.; Takegawa, K.; Esaki, N.
    Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase (2001), J. Bacteriol., 183, 2226-2233.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Heterologous expression renders Saccharomyces cerevisiae capable of utilization of D-Ala as a nitrogen source but also relieves the yeast from the toxicity of D-Ala Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
Sodium borohydride complete inactivation after dialysis against Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 D-Ala 37°C, pH 9.0 Schizosaccharomyces pombe
5
-
 L-Ala 37°C, pH 9.0 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O59828
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-Aminobutyrate 0.37% of the activity with L-Ala Schizosaccharomyces pombe D-2-Aminobutyrate
-
 r
L-Ala
-
 Schizosaccharomyces pombe D-Ala
-
 r
L-Ser 3.7% of the activity with L-Ala Schizosaccharomyces pombe D-Ser
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 D-Ala as substrate Schizosaccharomyces pombe
9.5
-
 L-Ala as substrate Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate coenzyme Schizosaccharomyces pombe