Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Shigella dysenteriae |
expression in Escherichia coli JM109 | Shigella boydii |
expression in Escherichia coli JM109 | Shigella flexneri |
expression in Escherichia coli JM109 | Shigella sonnei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium borohydride | reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation | Shigella boydii | |
Sodium borohydride | reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation | Shigella dysenteriae | |
Sodium borohydride | reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation | Shigella flexneri | |
Sodium borohydride | reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation | Shigella sonnei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
D-Ala | pH 8.3, 30°C | Shigella dysenteriae | |
20 | - |
L-Ala | pH 8.3, 30°C | Shigella dysenteriae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 43000, SDS-PAGE | Shigella dysenteriae |
43000 | - |
1 * 43000, SDS-PAGE | Shigella boydii |
43000 | - |
1 * 43000, SDS-PAGE | Shigella flexneri |
43000 | - |
1 * 43000, SDS-PAGE | Shigella sonnei |
46000 | - |
gel filtration | Shigella dysenteriae |
46000 | - |
gel filtration | Shigella boydii |
46000 | - |
gel filtration | Shigella flexneri |
46000 | - |
gel filtration | Shigella sonnei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shigella boydii | P0A6B6 | - |
- |
Shigella dysenteriae | Q932V0 | - |
- |
Shigella flexneri | P0A6B5 | - |
- |
Shigella sonnei | Q93HP9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Shigella dysenteriae |
- |
Shigella boydii |
- |
Shigella flexneri |
- |
Shigella sonnei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
209 | - |
L-Ala, 30°C | Shigella dysenteriae |
Storage Stability | Organism |
---|---|
-80°C, over 60 days | Shigella dysenteriae |
-80°C, over 60 days | Shigella boydii |
-80°C, over 60 days | Shigella flexneri |
-80°C, over 60 days | Shigella sonnei |
4°C, 3 weeks | Shigella dysenteriae |
4°C, 3 weeks | Shigella boydii |
4°C, 3 weeks | Shigella flexneri |
4°C, 3 weeks | Shigella sonnei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Shigella dysenteriae | D-Ala | - |
r | |
L-Ala | - |
Shigella boydii | D-Ala | - |
r | |
L-Ala | - |
Shigella flexneri | D-Ala | - |
r | |
L-Ala | - |
Shigella sonnei | D-Ala | - |
r | |
L-Ser | 2% of the activity with L-Ala | Shigella dysenteriae | D-Ser | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Shigella dysenteriae |
monomer | 1 * 43000, SDS-PAGE | Shigella boydii |
monomer | 1 * 43000, SDS-PAGE | Shigella flexneri |
monomer | 1 * 43000, SDS-PAGE | Shigella sonnei |
More | the monomeric enzyme interacts with other monomers in the presence of substrate | Shigella dysenteriae |
More | the monomeric enzyme interacts with other monomers in the presence of substrate | Shigella boydii |
More | the monomeric enzyme interacts with other monomers in the presence of substrate | Shigella flexneri |
More | the monomeric enzyme interacts with other monomers in the presence of substrate | Shigella sonnei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in presence or absence of pyridoxal 5'-phosphate | Shigella dysenteriae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate | Shigella dysenteriae |
40 | - |
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate | Shigella boydii |
40 | - |
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate | Shigella flexneri |
40 | - |
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate | Shigella sonnei |
50 | - |
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate | Shigella dysenteriae |
50 | - |
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate | Shigella boydii |
50 | - |
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate | Shigella flexneri |
50 | - |
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate | Shigella sonnei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Shigella dysenteriae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | cofactor | Shigella dysenteriae | |
pyridoxal 5'-phosphate | cofactor | Shigella boydii | |
pyridoxal 5'-phosphate | cofactor | Shigella flexneri | |
pyridoxal 5'-phosphate | cofactor | Shigella sonnei |