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Literature summary for 5.1.1.1 extracted from

  • Yokoigawa, K.; Hirasawa, R.; Ueno, H.; Okubo, Y.; Umesako, S.; Soda, K.
    Gene cloning and characterization of alanine racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei (2001), Biochem. Biophys. Res. Commun., 288, 676-684.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 Shigella dysenteriae
expression in Escherichia coli JM109 Shigella boydii
expression in Escherichia coli JM109 Shigella flexneri
expression in Escherichia coli JM109 Shigella sonnei

Inhibitors

Inhibitors Comment Organism Structure
Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella boydii
Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella dysenteriae
Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella flexneri
Sodium borohydride reduction of the enzyme by dialysis with sodium borohydride, the reduced enzyme is catalytically inactive and addition of pyridoxal 5'-phosphate does not reverse the inactivation Shigella sonnei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
D-Ala pH 8.3, 30°C Shigella dysenteriae
20
-
L-Ala pH 8.3, 30°C Shigella dysenteriae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
1 * 43000, SDS-PAGE Shigella dysenteriae
43000
-
1 * 43000, SDS-PAGE Shigella boydii
43000
-
1 * 43000, SDS-PAGE Shigella flexneri
43000
-
1 * 43000, SDS-PAGE Shigella sonnei
46000
-
gel filtration Shigella dysenteriae
46000
-
gel filtration Shigella boydii
46000
-
gel filtration Shigella flexneri
46000
-
gel filtration Shigella sonnei

Organism

Organism UniProt Comment Textmining
Shigella boydii P0A6B6
-
-
Shigella dysenteriae Q932V0
-
-
Shigella flexneri P0A6B5
-
-
Shigella sonnei Q93HP9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Shigella dysenteriae
-
Shigella boydii
-
Shigella flexneri
-
Shigella sonnei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
209
-
L-Ala, 30°C Shigella dysenteriae

Storage Stability

Storage Stability Organism
-80°C, over 60 days Shigella dysenteriae
-80°C, over 60 days Shigella boydii
-80°C, over 60 days Shigella flexneri
-80°C, over 60 days Shigella sonnei
4°C, 3 weeks Shigella dysenteriae
4°C, 3 weeks Shigella boydii
4°C, 3 weeks Shigella flexneri
4°C, 3 weeks Shigella sonnei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala
-
Shigella dysenteriae D-Ala
-
r
L-Ala
-
Shigella boydii D-Ala
-
r
L-Ala
-
Shigella flexneri D-Ala
-
r
L-Ala
-
Shigella sonnei D-Ala
-
r
L-Ser 2% of the activity with L-Ala Shigella dysenteriae D-Ser
-
r

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Shigella dysenteriae
monomer 1 * 43000, SDS-PAGE Shigella boydii
monomer 1 * 43000, SDS-PAGE Shigella flexneri
monomer 1 * 43000, SDS-PAGE Shigella sonnei
More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella dysenteriae
More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella boydii
More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella flexneri
More the monomeric enzyme interacts with other monomers in the presence of substrate Shigella sonnei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
in presence or absence of pyridoxal 5'-phosphate Shigella dysenteriae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella dysenteriae
40
-
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella boydii
40
-
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella flexneri
40
-
1 h, quick loss of activity in absence of pyridoxal 5'-phosphate Shigella sonnei
50
-
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella dysenteriae
50
-
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella boydii
50
-
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella flexneri
50
-
the enzyme is stabilized up to 50°C in presence of 0.4 mM pyridoxal 5'-phosphate Shigella sonnei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
Shigella dysenteriae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate cofactor Shigella dysenteriae
pyridoxal 5'-phosphate cofactor Shigella boydii
pyridoxal 5'-phosphate cofactor Shigella flexneri
pyridoxal 5'-phosphate cofactor Shigella sonnei