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Literature summary for 5.1.1.1 extracted from

  • Okubo, Y.; Yokoigawa, K.; Esaki, N.; Soda, K.; Kawai, H.
    Characterization of psychrophilic alanine racemase from Bacillus psychrosaccharolyticus (1999), Biochem. Biophys. Res. Commun., 256, 333-340.
    View publication on PubMed
Search for more literature for 5.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli SOLR with a plasmid yYOK3 Peribacillus psychrosaccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.2
-
 D-Ala 30°C, pH 8.3 Peribacillus psychrosaccharolyticus
17.9
-
 L-Ala 30°C, pH 8.3 Peribacillus psychrosaccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
 2 * 42500, SDS-PAGE Peribacillus psychrosaccharolyticus
73000
-
 gel filtration Peribacillus psychrosaccharolyticus

Organism

Organism UniProt Comment Textmining
Peribacillus psychrosaccharolyticus Q9S5V6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Peribacillus psychrosaccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
650
-
 L-Ala, 0°C Peribacillus psychrosaccharolyticus

Storage Stability

Storage Stability Organism
-80°C, 60 days Peribacillus psychrosaccharolyticus
4°C, 3 weeks Peribacillus psychrosaccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala
-
 Peribacillus psychrosaccharolyticus D-Ala
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 42500, SDS-PAGE Peribacillus psychrosaccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 in absence of pyridoxal 5'-phosphate Peribacillus psychrosaccharolyticus
60
-
 in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM Peribacillus psychrosaccharolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 60 about 30% of maximal activity at 0°C and at 60°C, in absence of pyridoxal 5'-phosphate Peribacillus psychrosaccharolyticus
30 50 30°C, 45% of maximal activity, 50°C: about 50% of maximal activity, in presence of an excess amount of pyridoxal 5'-phosphate, 0.4 mM Peribacillus psychrosaccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 8.3, 1 h, the purified is extremely labile over 35°C Peribacillus psychrosaccharolyticus
50
-
 pH 8.3, 1 h, stabilized up to 50°C in presence of excess amounts of pyridoxal 5'-phosphate, 0.4 mM, complete inactivation in absence of pyridoxal 5'-phosphate Peribacillus psychrosaccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
 Peribacillus psychrosaccharolyticus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.005 mM, at 30°C Peribacillus psychrosaccharolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Peribacillus psychrosaccharolyticus
-
-
 5.7