Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant gene which tandemly encodes the two polypeptides of the enzyme subunit, fragment 1 and fragment 2, cleaved at the position corresponding to the predicted hinge region. The mutant fragmentary alanine racemase is active at about 40% of the activity of the wild type enzyme | Geobacillus stearothermophilus |
General Stability | Organism |
---|---|
in 0.6 M to 1.5 M guanidine hydrochloride the dimeric enzyme is dissociated into a monomeric form, which is catalytically inactive | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | Geobacillus stearothermophilus | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | ? | - |
? | |
L-Ala | Bacillus sp. (in: Bacteria) | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | ? | - |
? | |
L-Ala | Bacillus sp. (in: Bacteria) YM-1 | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) YM-1 | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala | - |
Geobacillus stearothermophilus | D-Ala | - |
? | |
L-Ala | - |
Bacillus sp. (in: Bacteria) | D-Ala | - |
? | |
L-Ala | - |
Bacillus sp. (in: Bacteria) YM-1 | D-Ala | - |
? | |
L-Ala | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | Geobacillus stearothermophilus | ? | - |
? | |
L-Ala | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | Bacillus sp. (in: Bacteria) | ? | - |
? | |
L-Ala | enzyme provides D-Ala as an essential building block for biosynthesis of the peptidoglycan layer of the cell wall | Bacillus sp. (in: Bacteria) YM-1 | ? | - |
? |