Literature summary for 4.99.1.8 extracted from

Soni, A.; Goyal, M.; Prakash, K.; Bhardwaj, J.; Siddiqui, A.J.; Puri, S.K.
Cloning, expression and functional characterization of heme detoxification protein (HDP) from the rodent malaria parasite Plasmodium vinckei (2015), Gene, 566, 109-119 .

Search for more literature for 4.99.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Plasmodium vinckei petteri

Inhibitors

Inhibitors	Comment	Organism	Structure
Chloroquine	-	Plasmodium vinckei petteri
diethyldicarbonate	histidine modification using diethyldicarbonate leads to reduction in heme binding and hemozoin formation	Plasmodium vinckei petteri
mefloquine	-	Plasmodium vinckei petteri
additional information	not inhibitory up to 0.04 mM: pyrimethamine	Plasmodium vinckei petteri

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Plasmodium vinckei petteri	5829	-

Organism

Organism	UniProt	Comment	Textmining
Plasmodium vinckei petteri	F5BWX4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	enzyme is expressed throughout the intra erythrocytic stages	Plasmodium vinckei petteri	-
schizont	expression is increased in late trophozoite and schizont stages of parasite	Plasmodium vinckei petteri	-
trophozoite	expression is increased in late trophozoite and schizont stages of parasite	Plasmodium vinckei petteri	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
heme	enzyme binds heme with a very high affinity, KD value 89 nM	Plasmodium vinckei petteri	hemozoin	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	10 min, 70% residual activity	Plasmodium vinckei petteri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.2	-	assay at	Plasmodium vinckei petteri

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	5.5	-	Plasmodium vinckei petteri

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.01	-	pH 5.2, 37°C	Plasmodium vinckei petteri	Chloroquine
0.025	-	pH 5.2, 37°C	Plasmodium vinckei petteri	mefloquine

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Release 2023.1 (January 2023)