BRENDA - Enzyme Database show
show all sequences of 4.99.1.4

Characterization of the evolutionarily conserved iron-sulfur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana

Saha, K.; Webb, M.E.; Rigby, S.E.; Leech, H.K.; Warren, M.J.; Smith, A.G.; Biochem. J. 444, 227-237 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
C135A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
C199A
the mutant shows reduced specific activity compared to the wild type enzyme
Arabidopsis thaliana
C210A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
C213A
the mutant shows reduced specific activity compared to the wild type enzyme
Arabidopsis thaliana
C219A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 1.93 mol Fe2+ per mol of protein
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18600
-
2 * 18600, estimated from SDS-PAGE
Arabidopsis thaliana
36010
-
calculated from gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Arabidopsis thaliana
-
siroheme + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
33.25
-
mutant enzyme C199A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
36.08
-
mutant enzyme C213A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
38.5
-
wild type enzyme, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
43
-
mutant enzyme C219A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
44.67
-
mutant enzyme C135A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
55.17
-
mutant enzyme C210A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Fe2+
-
729160
Arabidopsis thaliana
siroheme + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 18600, estimated from SDS-PAGE
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C135A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
C199A
the mutant shows reduced specific activity compared to the wild type enzyme
Arabidopsis thaliana
C210A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
C213A
the mutant shows reduced specific activity compared to the wild type enzyme
Arabidopsis thaliana
C219A
the mutant shows increased specific activity compared to the wild type enzyme
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 1.93 mol Fe2+ per mol of protein
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
18600
-
2 * 18600, estimated from SDS-PAGE
Arabidopsis thaliana
36010
-
calculated from gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Arabidopsis thaliana
-
siroheme + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
33.25
-
mutant enzyme C199A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
36.08
-
mutant enzyme C213A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
38.5
-
wild type enzyme, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
43
-
mutant enzyme C219A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
44.67
-
mutant enzyme C135A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
55.17
-
mutant enzyme C210A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Fe2+
-
729160
Arabidopsis thaliana
siroheme + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 18600, estimated from SDS-PAGE
Arabidopsis thaliana
Other publictions for EC 4.99.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748869
Garai
Phylogenetic analysis and pho ...
Arabidopsis thaliana
Physiol. Mol. Biol. Plants
22
351-359
2016
-
-
-
-
-
-
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-
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1
-
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3
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3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
730419
Bali
Identification and characteriz ...
Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
-
-
1
1
-
-
-
-
-
-
1
1
-
1
-
-
1
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1
1
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1
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1
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1
1
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1
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729160
Saha
Characterization of the evolut ...
Arabidopsis thaliana
Biochem. J.
444
227-237
2012
-
-
1
-
5
-
-
-
1
1
2
1
-
5
-
-
1
-
-
1
6
-
1
1
-
-
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-
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-
-
1
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-
1
1
-
5
-
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1
1
2
1
-
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1
-
1
6
-
1
1
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-
-
-
-
-
-
-
-
716791
Romao
Evolution in a family of chela ...
Bacillus megaterium
Proc. Natl. Acad. Sci. USA
108
97-102
2011
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1
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1
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1
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1
-
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-
-
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-
-
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696120
Lobo
Functional characterization of ...
no activity in Desulfovibrio vulgaris
Biochem. J.
420
317-325
2009
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665692
Raux-Deery
Identification and characteriz ...
Arabidopsis thaliana
J. Biol. Chem.
280
4713-4721
2005
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1
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2
1
1
2
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4
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1
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-
2
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3
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1
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2
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1
1
2
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1
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1
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2
-
3
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649745
Raux
Identification and functional ...
Bacillus megaterium, Bacillus megaterium DSM 509
Biochem. J.
370
505-516
2003
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1
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2
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8
1
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1
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1
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1
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1
2
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1
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2
-
8
1
-
-
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1
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-
-
-
653297
Stroupe
CysG structure reveals tetrapy ...
Salmonella enterica
Nat. Struct. Biol.
10
1064-1073
2003
-
-
1
1
2
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1
-
1
1
-
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3
-
3
1
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1
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1
2
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1
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1
1
-
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3
-
3
1
-
-
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-
644283
Schubert
The structure of Saccharomyces ...
Saccharomyces cerevisiae
EMBO J.
21
2068-2075
2002
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1
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3
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2
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4
1
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1
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1
-
1
3
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2
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1
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2
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4
1
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1
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649817
Schubert
Structural diversity in metal ...
Saccharomyces cerevisiae
Biochem. Soc. Trans.
30
595-600
2002
-
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-
1
2
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1
-
1
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1
-
-
-
-
2
1
-
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1
2
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1
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2
1
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