BRENDA - Enzyme Database show
show all sequences of 4.99.1.4

Identification and characterization of the terminal enzyme of siroheme biosynthesis from Arabidopsis thaliana: a plastid-located sirohydrochlorin ferrochelatase containing a 2FE-2S center

Raux-Deery, E.; Leech, H.K.; Nakrieko, K.A.; McLean, K.J.; Munro, A.W.; Heathcote, P.; Rigby, S.E.; Smith, A.G.; Warren, M.J.; J. Biol. Chem. 280, 4713-4721 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Fe2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
it is not possible to measure an accurate KM value for the metals as at low concentration of metal the assay is not sensitive enough, whereas at higher metal ion concentrations the enzyme is inactivated
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Fe2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Arabidopsis thaliana
-
siroheme + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0054
-
isolated recombinant enzyme, with ferrous iron as substrate
Arabidopsis thaliana
0.0485
-
isolated recombinant enzyme, with cobalt as substrate
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Co2+
pH 8.0
665692
Arabidopsis thaliana
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Fe2+
-
665692
Arabidopsis thaliana
siroheme + H+
-
-
-
?
sirohydrochlorin + Fe2+
pH 8.0
665692
Arabidopsis thaliana
siroheme + H+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Fe2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
it is not possible to measure an accurate KM value for the metals as at low concentration of metal the assay is not sensitive enough, whereas at higher metal ion concentrations the enzyme is inactivated
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Fe2+
inactivates the enzyme at high concentrations
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Arabidopsis thaliana
-
siroheme + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0054
-
isolated recombinant enzyme, with ferrous iron as substrate
Arabidopsis thaliana
0.0485
-
isolated recombinant enzyme, with cobalt as substrate
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sirohydrochlorin + Co2+
pH 8.0
665692
Arabidopsis thaliana
cobalt-sirohydrochlorin + H+
-
-
-
?
sirohydrochlorin + Fe2+
-
665692
Arabidopsis thaliana
siroheme + H+
-
-
-
?
sirohydrochlorin + Fe2+
pH 8.0
665692
Arabidopsis thaliana
siroheme + H+
-
-
-
?
Other publictions for EC 4.99.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748869
Garai
Phylogenetic analysis and pho ...
Arabidopsis thaliana
Physiol. Mol. Biol. Plants
22
351-359
2016
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-
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1
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3
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3
-
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-
-
1
-
-
1
-
-
730419
Bali
Identification and characteriz ...
Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
-
-
1
1
-
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1
1
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1
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1
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1
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1
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729160
Saha
Characterization of the evolut ...
Arabidopsis thaliana
Biochem. J.
444
227-237
2012
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1
-
5
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1
1
2
1
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5
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1
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1
6
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1
1
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1
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1
1
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5
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1
1
2
1
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1
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1
6
-
1
1
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716791
Romao
Evolution in a family of chela ...
Bacillus megaterium
Proc. Natl. Acad. Sci. USA
108
97-102
2011
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1
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696120
Lobo
Functional characterization of ...
no activity in Desulfovibrio vulgaris
Biochem. J.
420
317-325
2009
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1
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665692
Raux-Deery
Identification and characteriz ...
Arabidopsis thaliana
J. Biol. Chem.
280
4713-4721
2005
-
-
1
-
-
-
2
1
1
2
-
1
-
4
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1
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2
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3
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1
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3
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649745
Raux
Identification and functional ...
Bacillus megaterium, Bacillus megaterium DSM 509
Biochem. J.
370
505-516
2003
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1
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1
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1
2
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4
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1
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8
1
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1
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8
1
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1
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653297
Stroupe
CysG structure reveals tetrapy ...
Salmonella enterica
Nat. Struct. Biol.
10
1064-1073
2003
-
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1
1
2
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1
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1
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3
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1
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1
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3
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3
1
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644283
Schubert
The structure of Saccharomyces ...
Saccharomyces cerevisiae
EMBO J.
21
2068-2075
2002
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1
1
3
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2
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2
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1
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4
1
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3
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1
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4
1
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1
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649817
Schubert
Structural diversity in metal ...
Saccharomyces cerevisiae
Biochem. Soc. Trans.
30
595-600
2002
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1
2
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1
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1
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2
1
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1
2
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1
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