BRENDA - Enzyme Database show
show all sequences of 4.99.1.4

Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium

Raux, E.; Leech, H.K.; Beck, R.; Schubert, H.L.; Santander, P.J.; Roessner, C.A.; Scott, A.I.; Martens, J.H.; Jahn, D.; Thermes, C.; Rambach, A.; Warren, M.J.; Biochem. J. 370, 505-516 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
sirB, expression in Escherichia coli BL21(DE3)pLysS, sirA,B,C operon, amino acid sequence
Bacillus megaterium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
recombinant SirB
Bacillus megaterium
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
1 * 33000, SDS-PAGE
Bacillus megaterium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Bacillus megaterium
SirB is responsible for the final step in siroheme synthesis
siroheme + 2 H+
-
Bacillus megaterium
?
sirohydrochlorin + Fe2+
Bacillus megaterium DSM 509
SirB is responsible for the final step in siroheme synthesis
siroheme + 2 H+
-
Bacillus megaterium DSM 509
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
strain DSM509
-
Bacillus megaterium DSM 509
-
strain DSM509
-
Purification (Commentary)
Commentary
Organism
recombinant SirB
Bacillus megaterium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0034
-
pH 8, cobaltochelation of precorrin-2
Bacillus megaterium
0.337
-
pH 8, cobaltochelation of sirohydrochlorin
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
precorrin-2 + Co2+
SirB, much lower specific activity than with sirohydrochlorin
649745
Bacillus megaterium
cobalt-precorrin-2 + 2 H+
-
649745
Bacillus megaterium
?
precorrin-2 + Co2+
SirB, much lower specific activity than with sirohydrochlorin
649745
Bacillus megaterium DSM 509
cobalt-precorrin-2 + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Co2+
SirB, lower specificity for cobalt than for iron
649745
Bacillus megaterium
cobalt-sirohydrochlorin + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Co2+
SirB, lower specificity for cobalt than for iron
649745
Bacillus megaterium DSM 509
cobalt-sirohydrochlorin + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Fe2+
SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt
649745
Bacillus megaterium
siroheme + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Fe2+
SirB is responsible for the final step in siroheme synthesis
649745
Bacillus megaterium
siroheme + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Fe2+
SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt
649745
Bacillus megaterium DSM 509
siroheme + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Fe2+
SirB is responsible for the final step in siroheme synthesis
649745
Bacillus megaterium DSM 509
siroheme + 2 H+
-
649745
Bacillus megaterium DSM 509
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
sirB, expression in Escherichia coli BL21(DE3)pLysS, sirA,B,C operon, amino acid sequence
Bacillus megaterium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
recombinant SirB
Bacillus megaterium
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
1 * 33000, SDS-PAGE
Bacillus megaterium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sirohydrochlorin + Fe2+
Bacillus megaterium
SirB is responsible for the final step in siroheme synthesis
siroheme + 2 H+
-
Bacillus megaterium
?
sirohydrochlorin + Fe2+
Bacillus megaterium DSM 509
SirB is responsible for the final step in siroheme synthesis
siroheme + 2 H+
-
Bacillus megaterium DSM 509
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant SirB
Bacillus megaterium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0034
-
pH 8, cobaltochelation of precorrin-2
Bacillus megaterium
0.337
-
pH 8, cobaltochelation of sirohydrochlorin
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
precorrin-2 + Co2+
SirB, much lower specific activity than with sirohydrochlorin
649745
Bacillus megaterium
cobalt-precorrin-2 + 2 H+
-
649745
Bacillus megaterium
?
precorrin-2 + Co2+
SirB, much lower specific activity than with sirohydrochlorin
649745
Bacillus megaterium DSM 509
cobalt-precorrin-2 + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Co2+
SirB, lower specificity for cobalt than for iron
649745
Bacillus megaterium
cobalt-sirohydrochlorin + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Co2+
SirB, lower specificity for cobalt than for iron
649745
Bacillus megaterium DSM 509
cobalt-sirohydrochlorin + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Fe2+
SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt
649745
Bacillus megaterium
siroheme + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Fe2+
SirB is responsible for the final step in siroheme synthesis
649745
Bacillus megaterium
siroheme + 2 H+
-
649745
Bacillus megaterium
?
sirohydrochlorin + Fe2+
SirB, monofunctional ferrochelatase, higher specificity for iron over cobalt
649745
Bacillus megaterium DSM 509
siroheme + 2 H+
-
649745
Bacillus megaterium DSM 509
?
sirohydrochlorin + Fe2+
SirB is responsible for the final step in siroheme synthesis
649745
Bacillus megaterium DSM 509
siroheme + 2 H+
-
649745
Bacillus megaterium DSM 509
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Bacillus megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus megaterium
Other publictions for EC 4.99.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748869
Garai
Phylogenetic analysis and pho ...
Arabidopsis thaliana
Physiol. Mol. Biol. Plants
22
351-359
2016
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1
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3
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3
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1
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1
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730419
Bali
Identification and characteriz ...
Paracoccus pantotrophus
Mol. Microbiol.
92
153-163
2014
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1
1
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1
1
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1
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1
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1
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1
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729160
Saha
Characterization of the evolut ...
Arabidopsis thaliana
Biochem. J.
444
227-237
2012
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1
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5
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1
1
2
1
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5
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1
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1
6
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1
1
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1
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1
1
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5
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1
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2
1
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1
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1
6
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1
1
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716791
Romao
Evolution in a family of chela ...
Bacillus megaterium
Proc. Natl. Acad. Sci. USA
108
97-102
2011
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1
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1
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696120
Lobo
Functional characterization of ...
no activity in Desulfovibrio vulgaris
Biochem. J.
420
317-325
2009
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1
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665692
Raux-Deery
Identification and characteriz ...
Arabidopsis thaliana
J. Biol. Chem.
280
4713-4721
2005
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1
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2
1
1
2
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1
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4
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1
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2
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3
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1
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1
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649745
Raux
Identification and functional ...
Bacillus megaterium, Bacillus megaterium DSM 509
Biochem. J.
370
505-516
2003
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1
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1
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1
2
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4
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1
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2
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8
1
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1
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1
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1
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1
2
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1
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2
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8
1
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1
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653297
Stroupe
CysG structure reveals tetrapy ...
Salmonella enterica
Nat. Struct. Biol.
10
1064-1073
2003
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-
1
1
2
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-
-
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1
-
1
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1
1
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3
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3
1
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1
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1
2
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1
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1
1
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3
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3
1
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644283
Schubert
The structure of Saccharomyces ...
Saccharomyces cerevisiae
EMBO J.
21
2068-2075
2002
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-
1
1
3
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-
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2
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2
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1
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2
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4
1
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1
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1
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1
3
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2
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1
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2
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4
1
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1
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649817
Schubert
Structural diversity in metal ...
Saccharomyces cerevisiae
Biochem. Soc. Trans.
30
595-600
2002
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-
1
2
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1
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1
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1
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2
1
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1
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