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Literature summary for 4.99.1.3 extracted from

  • Leech, H.K.; Raux, E.; McLean, K.J.; Munro, A.W.; Robinson, N.J.; Borrelly, G.P.; Malten, M.; Jahn, D.; Rigby, S.E.; Heathcote, P.; Warren, M.J.
    Characterization of the cobaltochelatase CbiXL: evidence for a 4Fe-4S center housed within an MXCXXC motif (2003), J. Biol. Chem., 278, 41900-41907.
    View publication on PubMed
Search for more literature for 4.99.1.3

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli and Bacillus megaterium Priestia megaterium
overexpressed in Escherichia coli Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
C259S site-directed mutagenesis Priestia megaterium
C262S site-directed mutagenesis Priestia megaterium
M257L site-directed mutagenesis Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur center Priestia megaterium
Fe2+ iron-sulfur center Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Priestia megaterium
38000
-
 SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Co2+ Priestia megaterium biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ Synechocystis sp. biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ Priestia megaterium DSM 509 biosynthesis of vitamin B12 cobalt-sirohydrochlorin + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 strain DSM509
-
Priestia megaterium DSM 509
-
 strain DSM509
-
Synechocystis sp.
-
 strain PCC6803
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium
-
 Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 overproduced in Escherichia coli Synechocystis sp.
1.4
-
 CbiX6H, overproduced in Escherichia coli Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Co2+
-
 Priestia megaterium cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+
-
 Synechocystis sp. cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Priestia megaterium cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Synechocystis sp. cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+
-
 Priestia megaterium DSM 509 cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Co2+ biosynthesis of vitamin B12 Priestia megaterium DSM 509 cobalt-sirohydrochlorin + H+
-
 ?

Synonyms

Synonyms Comment Organism
CbiX
-
 Priestia megaterium
CbiX
-
 Synechocystis sp.
CbiX0H
-
 Priestia megaterium
CbiXL
-
 Priestia megaterium
CbiXL
-
 Synechocystis sp.
CbiXOH
-
 Synechocystis sp.
cobaltochelatase
-
 Priestia megaterium
cobaltochelatase
-
 Synechocystis sp.