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Literature summary for 4.99.1.10 extracted from
- Re-examination of Mg-dechelation reaction in the degradation of chlorophylls using chlorophyllin a as a substrate (2002), Photosynth. Res., 74, 217-223 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 111% activity at 1 mM | Chenopodium album |  |
| histidine | 152% activity at 1 mM | Chenopodium album | |
| Tiron | 185% activity at 1 mM | Chenopodium album | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroquinone | 11% residual activity at 1 mM | Chenopodium album | |
| KCN | 19% residual activity at 1 mM | Chenopodium album | |
| additional information | not influenced by NaN3 | Chenopodium album | |
| propyl gallate | 4% residual activity at 1 mM | Chenopodium album | |
| Sodium ascorbate | complete inhibition at 1 mM | Chenopodium album | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000951 | - |
chlorophyllin a | at pH 7.5 and 30°C | Chenopodium album | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Chenopodium album |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chenopodium album | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE -Toyopearl column chromatography, butyl column chromatography. and HW-55 gel filtration | Chenopodium album |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Chenopodium album | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000000004 | - |
crude extract, at pH 7.5 and 30°C | Chenopodium album |
| 0.00000105 | - |
after 28fold purification, at pH 7.5 and 30°C | Chenopodium album |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyllin a + 2 H+ | - |
Chenopodium album | pheophorbin a + Mg2+ | - |
? | |
| additional information | the enzyme is not active with chlorophyllide a | Chenopodium album | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mg-dechelatase | - |
Chenopodium album |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the purified protein is relatively heat stable and loses half of its activity after 10 min at 55°C | Chenopodium album |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chenopodium album |
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Release 2023.1 (January 2023)
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