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Literature summary for 4.98.1.1 extracted from

  • Abril, B.; Sanchez-Torres, E.A.; Bou, R.; Garcia-Perez, J.V.; Benedito, J.
    Ultrasound intensification of ferrochelatase extraction from pork liver as a strategy to improve ZINC-protoporphyrin formation (2021), Ultrason. Sonochem., 78, 105703.
    View publication on PubMed
Search for more literature for 4.98.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information ultrasound application during FeCH extraction leads to an improved enzymatic activity and further increase in the formation of Zn-protoporphyrin (ZnPP) method of increasing the enzyme catalysis, optimization, overview. Improvement of the rate of ZnPP formation with ultrasound extraction by up to 33.3% compared to conventional extraction Sus scrofa

Protein Variants

Protein Variants Comment Organism
additional information ultrasound intensification of ferrochelatase extraction from pork liver as a strategy to improve Zn-protoporphyrin formation. Ultrasound application during FeCH extraction leads to an improved enzymatic activity and further increase in the formation of ZnPP, method optimization, overview Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of ZnPP formation using a FeCH extract from pork liver obtained by conventional and continuous ultrasound extraction for 5 min, the latter resulting in higher activity. And kinetics of ZnPP formation using a FeCH extract from pork liver obtained by continuous ultrasound extraction (US-C) and pulsed ultrasound extraction, the first resulting in higher activity Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin + Fe2+ Sus scrofa
-
 protoheme + 2 H+
-
 ?
protoporphyrin + Zn2+ Sus scrofa
-
 Zn-protoporphyrin + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa A0A287B3T2
-
-

Purification (Commentary)

Purification (Comment) Organism
usage of high power ultrasound in continuous and pulsed modes to intensify the extraction of the enzyme FeCH from pork liver Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific enzymatic activity is 7.6 nmol of ZnPP/g dry matter * min and the product forms at 120 min of 684 nmol/l ZnPP, stationary reaction phase measurement Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Zn-protoporphyrin (ZnPP) is quantified by fluorescence measurements as the product of the reaction due to its ability to emit fluorescence (unlike myoglobin and nitrosylmyoglobin) with excitation and emission peaks at around 420 nm and 590 nm, respectively Sus scrofa ?
-
-
protoporphyrin + Fe2+
-
 Sus scrofa protoheme + 2 H+
-
 ?
protoporphyrin + Zn2+
-
 Sus scrofa Zn-protoporphyrin + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
FeCH
-
 Sus scrofa
ferrochelatase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sus scrofa

General Information

General Information Comment Organism
physiological function the enzyme ferrochelatase (FeCH), which is naturally present in pork liver, catalyses the formation of Zinc-protoporphyrin (ZnPP), a natural pigment responsible for the typical color of dry-cured Italian Parma ham Sus scrofa