Literature summary for 4.98.1.1 extracted from

Medlock, A.E.; Najahi-Missaoui, W.; Shiferaw, M.T.; Albetel, A.N.; Lanzilotta, W.N.; Dailey, H.A.
Insight into the function of active site residues in the catalytic mechanism of human ferrochelatase (2021), Biochem. J., 478, 3239-3252 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzymes E343D and E343Q in the presence of cobalt chloride, hanging drop vapor diffusion method, using 0.1 M Bis-Tris pH 6.5, 0.05 M ammonium sulfate and a range of pentaerythritol ethoxylate (15/4 EO/OH) varying from 20% to 35% (w/v)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E343D	the catalytic efficiency of the variant is significantly diminished from the wild type enzyme	Homo sapiens
E343Q	the mutant has unmeasurable activity in vitro with iron and protoporphyrin as substrates	Homo sapiens
F110A/E343D	the catalytic efficiency of the variant is significantly diminished from the wild type enzyme	Homo sapiens
K304L	the mutant shows 7% activity compared to the wild type enzyme	Homo sapiens
M76A	inactive	Homo sapiens
M76H	the mutant has a higher affinity for heme relative to the wild type enzyme	Homo sapiens
M76Y	the variant exhibits decreased kcat, as well as KM for mesoporphyrin IX and Km for iron as compared with the wild-type enzyme. The mutant is not able to utilize copper as a substrate in the in vitro	Homo sapiens
Q302A	the mutant shows 40% activity compared to the wild type enzyme	Homo sapiens
Q302D	the mutant's enzyme activity is below detection limit	Homo sapiens
S303A	the mutant shows 47% activity compared to the wild type enzyme	Homo sapiens
S303P	the mutant's enzyme activity is below detection limit	Homo sapiens
S303T	the mutant's enzyme activity is below detection limit	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P22830	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
mesoporphyrin IX + Co2+	-	Homo sapiens	cobalt mesoporphyrin IX + 2 H+	-	?
mesoporphyrin IX + Fe2+	-	Homo sapiens	iron mesoporphyrin IX + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
ferrochelatase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens

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Release 2023.1 (January 2023)