Any feedback?
Literature summary for 4.98.1.1 extracted from
- Metal ion selectivity and substrate inhibition in the metal ion chelation catalyzed by human ferrochelatase (2009), J. Biol. Chem., 284, 33795-33799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deuteroporphyrin IX | - |
Homo sapiens |  |
| additional information | no inhibition by deuteroporphyrin | Homo sapiens | |
| porphyrin | high porphyrin concentrations noticeably increase the extent of zinc inhibition | Homo sapiens | |
| Zn2+ | uncompetitive substrate inhibitor, zinc inhibition is enhanced by increasing porphyrin concentration, zinc inhibits by binding to an enzyme-product complex and is likely to be the second substrate in an ordered mechanism, this inhibition is not observed in the presence of higher concentrations of the detergent cholate, and it is likely that increasing detergent concentrations also increases KM for deuteroporphyrin Ix as substrate and so reduces the concentration of enzymeproduct species for the inhibitor to bind | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
Zn2+ | for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 0.0015 | - |
Zn2+ | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 0.0026 | - |
deuteroporphyrin IX | for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 0.0461 | - |
deuteroporphyrin IX | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc is an effective inhibitor of the reaction as well as being a substrate | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin IX + Fe2+ | Homo sapiens | - |
protoheme IX + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deuteroporphyrin IX + Zn2+ | in vitro, zinc is the preferred substrate at all concentrations of porphyrin | Homo sapiens | Zn deuteroporphyrin IX + H+ | - |
? | |
| protoporphyrin IX + Fe2+ | - |
Homo sapiens | protoheme IX + 2 H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FeCH | - |
Homo sapiens |
| ferrochelatase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
Zn2+ | no added cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 3.5 | - |
Zn2+ | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0712 | - |
deuteroporphyrin IX | for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 0.3376 | - |
deuteroporphyrin IX | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
deuteroporphyrin IX | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 1200 | - |
deuteroporphyrin IX | for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 2200 | - |
Zn2+ | 0.5% cholate, for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
| 19000 | - |
Zn2+ | for the human ferrochelatase (R115L)-catalyzed insertion of zinc into deuteroporphyrin | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
