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Literature summary for 4.98.1.1 extracted from

  • Shepherd, M.; Dailey, T.A.; Dailey, H.A.
    A new class of [2Fe-2S]-cluster-containing protoporphyrin (IX) ferrochelatases (2006), Biochem. J., 397, 47-52.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information convergent evolution in prokaryotes Pseudomonas aeruginosa
additional information convergent evolution in prokaryotes Pseudomonas putida
additional information convergent evolution in prokaryotes Mycobacterium tuberculosis
additional information convergent evolution in prokaryotes Azotobacter vinelandii
additional information convergent evolution in prokaryotes Myxococcus xanthus
additional information convergent evolution in prokaryotes Bdellovibrio bacteriovorus
additional information convergent evolution in prokaryotes Porphyromonas gingivalis
additional information convergent evolution in prokaryotes, possesses a cysteine-rich C-terminal extension similar to that of the human enzyme Caulobacter vibrioides

Cloned(Commentary)

Cloned (Comment) Organism
-
Mycobacterium tuberculosis
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant Pseudomonas aeruginosa
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant Pseudomonas putida
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant Myxococcus xanthus
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant Bdellovibrio bacteriovorus
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant Porphyromonas gingivalis
into the expression vector pTrcHisA, not expressed in Escherichia coli hemH deletion mutant Azotobacter vinelandii

Protein Variants

Protein Variants Comment Organism
C213S loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain Myxococcus xanthus
C219S loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain Myxococcus xanthus
C220S loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain Myxococcus xanthus
C230S loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain Myxococcus xanthus
C341S loss of the [2Fe-2S] cluster Caulobacter vibrioides
D207C does not form a [2Fe-2S] cluster Pseudomonas putida
delta209–230 retains enough activity to complement Escherichia coli deltahemH, but is unstable after purification Myxococcus xanthus
K202T/D207C does not form a [2Fe-2S] cluster Pseudomonas putida
K202T/deltaV204/D207C does not form a [2Fe-2S] cluster Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
Fe2+ mutant C341S Caulobacter vibrioides
0.0047
-
mesoporphyrin IX mutant C341S Caulobacter vibrioides
0.0065
-
Fe2+ wild-type Myxococcus xanthus
0.008
-
mesoporphyrin IX
-
Bdellovibrio bacteriovorus
0.0087
-
mesoporphyrin IX wild-type Caulobacter vibrioides
0.0089
-
mesoporphyrin IX
-
Pseudomonas putida
0.0096
-
mesoporphyrin IX wild-type Myxococcus xanthus
0.011
-
Fe2+ wild-type Caulobacter vibrioides
0.0127
-
mesoporphyrin IX mutant C219S Myxococcus xanthus
0.0139
-
Fe2+ mutant C219S Myxococcus xanthus
0.02
-
Fe2+
-
Pseudomonas putida
0.028
-
Fe2+
-
Bdellovibrio bacteriovorus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains [2Fe-2S] cluster Mycobacterium tuberculosis
Fe2+ contains a [2Fe-2S] cluster Caulobacter vibrioides
Fe2+ contains [2Fe-2S] cluster that is coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity Myxococcus xanthus
Fe2+ contains [2Fe-2S] clusters that are coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity Azotobacter vinelandii
Fe2+ contains [2Fe-2S] clusters that are coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity Bdellovibrio bacteriovorus
Fe2+ no [2Fe-2S] cluster, contains three of the four cluster-ligating cysteine residues Pseudomonas putida
additional information no [2Fe-2S] cluster Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Bdellovibrio bacteriovorus
-
-
-
Caulobacter vibrioides
-
-
-
Mycobacterium tuberculosis
-
-
-
Myxococcus xanthus
-
-
-
Porphyromonas gingivalis Q7MXP4
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida
-
Mycobacterium tuberculosis
-
Myxococcus xanthus
-
Caulobacter vibrioides
-
Bdellovibrio bacteriovorus
precipitates immediately after purification, making it unsuitable for further analysis Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mesoporphyrin + Fe2+
-
Mycobacterium tuberculosis mesoheme + H+
-
?
mesoporphyrin + Fe2+
-
Myxococcus xanthus mesoheme + H+
-
?
mesoporphyrin + Fe2+
-
Bdellovibrio bacteriovorus mesoheme + H+
-
?
mesoporphyrin IX + Fe2+
-
Pseudomonas putida Fe-mesoporphyrin IX + 2 H+
-
?
mesoporphyrin IX + Fe2+
-
Caulobacter vibrioides Fe-mesoporphyrin IX + 2 H+
-
?
mesoporphyrin IX + Fe2+
-
Myxococcus xanthus mesoheme + 2 H+
-
?
mesoporphyrin IX + Fe2+
-
Bdellovibrio bacteriovorus mesoheme + 2 H+
-
?

Synonyms

Synonyms Comment Organism
protoporphyrin (IX) ferrochelatase
-
Pseudomonas aeruginosa
protoporphyrin (IX) ferrochelatase
-
Pseudomonas putida
protoporphyrin (IX) ferrochelatase
-
Mycobacterium tuberculosis
protoporphyrin (IX) ferrochelatase
-
Azotobacter vinelandii
protoporphyrin (IX) ferrochelatase
-
Myxococcus xanthus
protoporphyrin (IX) ferrochelatase
-
Caulobacter vibrioides
protoporphyrin (IX) ferrochelatase
-
Bdellovibrio bacteriovorus
protoporphyrin (IX) ferrochelatase
-
Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
Fe2+ mutant C219S Myxococcus xanthus
0.063
-
Fe2+ mutant C341S Caulobacter vibrioides
0.073
-
Fe2+
-
Bdellovibrio bacteriovorus
0.08167
-
Fe2+ wild-type Myxococcus xanthus
0.195
-
Fe2+
-
Pseudomonas putida
0.267
-
Fe2+ wild-type Caulobacter vibrioides