Application | Comment | Organism |
---|---|---|
additional information | convergent evolution in prokaryotes | Pseudomonas aeruginosa |
additional information | convergent evolution in prokaryotes | Pseudomonas putida |
additional information | convergent evolution in prokaryotes | Mycobacterium tuberculosis |
additional information | convergent evolution in prokaryotes | Azotobacter vinelandii |
additional information | convergent evolution in prokaryotes | Myxococcus xanthus |
additional information | convergent evolution in prokaryotes | Bdellovibrio bacteriovorus |
additional information | convergent evolution in prokaryotes | Porphyromonas gingivalis |
additional information | convergent evolution in prokaryotes, possesses a cysteine-rich C-terminal extension similar to that of the human enzyme | Caulobacter vibrioides |
Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant | Pseudomonas aeruginosa |
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant | Pseudomonas putida |
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant | Myxococcus xanthus |
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant | Bdellovibrio bacteriovorus |
into the expression vector pTrcHisA, expressed in Escherichia coli hemH deletion mutant | Porphyromonas gingivalis |
into the expression vector pTrcHisA, not expressed in Escherichia coli hemH deletion mutant | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
C213S | loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain | Myxococcus xanthus |
C219S | loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain | Myxococcus xanthus |
C220S | loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain | Myxococcus xanthus |
C230S | loss of the [2Fe-2S] cluster, able to support the growth of the Escherichia coli deltahemH strain | Myxococcus xanthus |
C341S | loss of the [2Fe-2S] cluster | Caulobacter vibrioides |
D207C | does not form a [2Fe-2S] cluster | Pseudomonas putida |
delta209230 | retains enough activity to complement Escherichia coli deltahemH, but is unstable after purification | Myxococcus xanthus |
K202T/D207C | does not form a [2Fe-2S] cluster | Pseudomonas putida |
K202T/deltaV204/D207C | does not form a [2Fe-2S] cluster | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
Fe2+ | mutant C341S | Caulobacter vibrioides | |
0.0047 | - |
mesoporphyrin IX | mutant C341S | Caulobacter vibrioides | |
0.0065 | - |
Fe2+ | wild-type | Myxococcus xanthus | |
0.008 | - |
mesoporphyrin IX | - |
Bdellovibrio bacteriovorus | |
0.0087 | - |
mesoporphyrin IX | wild-type | Caulobacter vibrioides | |
0.0089 | - |
mesoporphyrin IX | - |
Pseudomonas putida | |
0.0096 | - |
mesoporphyrin IX | wild-type | Myxococcus xanthus | |
0.011 | - |
Fe2+ | wild-type | Caulobacter vibrioides | |
0.0127 | - |
mesoporphyrin IX | mutant C219S | Myxococcus xanthus | |
0.0139 | - |
Fe2+ | mutant C219S | Myxococcus xanthus | |
0.02 | - |
Fe2+ | - |
Pseudomonas putida | |
0.028 | - |
Fe2+ | - |
Bdellovibrio bacteriovorus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains [2Fe-2S] cluster | Mycobacterium tuberculosis | |
Fe2+ | contains a [2Fe-2S] cluster | Caulobacter vibrioides | |
Fe2+ | contains [2Fe-2S] cluster that is coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity | Myxococcus xanthus | |
Fe2+ | contains [2Fe-2S] clusters that are coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity | Azotobacter vinelandii | |
Fe2+ | contains [2Fe-2S] clusters that are coordinated by a group of four cysteine residues contained in an internal amino acid segment of ca. 20 residues in length, role for the [2Fe-2S] cluster in iron affinity | Bdellovibrio bacteriovorus | |
Fe2+ | no [2Fe-2S] cluster, contains three of the four cluster-ligating cysteine residues | Pseudomonas putida | |
additional information | no [2Fe-2S] cluster | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Bdellovibrio bacteriovorus | - |
- |
- |
Caulobacter vibrioides | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Myxococcus xanthus | - |
- |
- |
Porphyromonas gingivalis | Q7MXP4 | - |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
- |
Mycobacterium tuberculosis |
- |
Myxococcus xanthus |
- |
Caulobacter vibrioides |
- |
Bdellovibrio bacteriovorus |
precipitates immediately after purification, making it unsuitable for further analysis | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesoporphyrin + Fe2+ | - |
Mycobacterium tuberculosis | mesoheme + H+ | - |
? | |
mesoporphyrin + Fe2+ | - |
Myxococcus xanthus | mesoheme + H+ | - |
? | |
mesoporphyrin + Fe2+ | - |
Bdellovibrio bacteriovorus | mesoheme + H+ | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Pseudomonas putida | Fe-mesoporphyrin IX + 2 H+ | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Caulobacter vibrioides | Fe-mesoporphyrin IX + 2 H+ | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Myxococcus xanthus | mesoheme + 2 H+ | - |
? | |
mesoporphyrin IX + Fe2+ | - |
Bdellovibrio bacteriovorus | mesoheme + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protoporphyrin (IX) ferrochelatase | - |
Pseudomonas aeruginosa |
protoporphyrin (IX) ferrochelatase | - |
Pseudomonas putida |
protoporphyrin (IX) ferrochelatase | - |
Mycobacterium tuberculosis |
protoporphyrin (IX) ferrochelatase | - |
Azotobacter vinelandii |
protoporphyrin (IX) ferrochelatase | - |
Myxococcus xanthus |
protoporphyrin (IX) ferrochelatase | - |
Caulobacter vibrioides |
protoporphyrin (IX) ferrochelatase | - |
Bdellovibrio bacteriovorus |
protoporphyrin (IX) ferrochelatase | - |
Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Fe2+ | mutant C219S | Myxococcus xanthus | |
0.063 | - |
Fe2+ | mutant C341S | Caulobacter vibrioides | |
0.073 | - |
Fe2+ | - |
Bdellovibrio bacteriovorus | |
0.08167 | - |
Fe2+ | wild-type | Myxococcus xanthus | |
0.195 | - |
Fe2+ | - |
Pseudomonas putida | |
0.267 | - |
Fe2+ | wild-type | Caulobacter vibrioides |