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Literature summary for 4.98.1.1 extracted from

  • Ohgari, Y.; Sawamoto, M.; Yamamoto, M.; Kohno, H.; Taketani, S.
    Ferrochelatase consisting of wild-type and mutated subunits from patients with a dominant-inherited disease, erythropoietic protoporphyria, is an active but unstable dimer (2005), Hum. Mol. Genet., 14, 327-334.
    View publication on PubMed
Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C395delta co-expressed with wild-type, 60% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified Homo sapiens
H157A co-expressed with wild-type, 23-30% of wild-type activity (homogenate), loss of function (purified enzyme) Homo sapiens
H263A co-expressed with wild-type, 23-30% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified Homo sapiens
H388A co-expressed with wild-type, 23-30% of wild-type activity (homogenate), 37.5-43% of wild-type activity (purified enzyme), exhibits a heterodimeric form when purified Homo sapiens
I186T causes erythropoietic protoporphyria, co-expressed with wild-type, no activity in the homogenate Homo sapiens
M267I causes erythropoietic protoporphyria, co-expressed with wild-type, no activity in the homogenate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 mesoporphyrin mutant H388A, 37°C Homo sapiens
0.0085
-
 mesoporphyrin wild-type, 37°C Homo sapiens
0.0092
-
 mesoporphyrin mutant H388A, co-expressed with wild-type, 37°C Homo sapiens
0.0093
-
 mesoporphyrin mutant C395delta, co-expressed with wild-type, 37°C Homo sapiens
0.0095
-
 Zn2+ mutant H263A, co-expressed with wild-type, 37°C Homo sapiens
0.0105
-
 Zn2+ mutant H388A, co-expressed with wild-type, 37°C Homo sapiens
0.0105
-
 mesoporphyrin mutant M267I, co-expressed with wild-type, 37°C Homo sapiens
0.0108
-
 Zn2+ mutant H388A, 37°C Homo sapiens
0.011
-
 Zn2+ mutant I186T, co-expressed with wild-type, 37°C Homo sapiens
0.0111
-
 mesoporphyrin mutant I186T, co-expressed with wild-type, 37°C Homo sapiens
0.0114
-
 Zn2+ wild-type, 37°C Homo sapiens
0.012
-
 mesoporphyrin mutant H263A, 37°C Homo sapiens
0.012
-
 mesoporphyrin mutant H263A, co-expressed with wild-type, 37°C Homo sapiens
0.018
-
 Zn2+ mutant C395delta, co-expressed with wild-type, 37°C Homo sapiens
0.024
-
 Zn2+ mutant M267I, co-expressed with wild-type, 37°C Homo sapiens
0.04
-
 Zn2+ mutant H263A, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE, gel filtration, wild-type Homo sapiens
80000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin + Fe2+ Homo sapiens
-
 protoheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22830
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mesoporphyrin + Zn2+ 37°C Homo sapiens Zn-mesoporphyrin + 2 H+
-
 ?
protoporphyrin + Fe2+
-
 Homo sapiens protoheme + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE, gel filtration, wild-type Homo sapiens

Synonyms

Synonyms Comment Organism
ferrochelatase
-
 Homo sapiens