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Literature summary for 4.98.1.1 extracted from

  • Najahi-Missaoui, W.; Dailey, H.A.
    Production and characterization of erythropoietic protoporphyric heterodimeric ferrochelatases (2005), Blood, 106, 1098-1104.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C406S complements the ferrochelatase-deficient delta-hemH Escherichia coli, no activity Homo sapiens
F417S complements the ferrochelatase-deficient delta-hemH Escherichia coli, no activity Homo sapiens
H386P does not complement the ferrochelatase-deficient delta-hemH Escherichia coli, the heterodimeric form (co-expressed with wild-type) cannot be isolated as pure protein, no activity Homo sapiens
M288K does not complement the ferrochelatase-deficient delta-hemH Escherichia coli, the heterodimeric form (co-expressed with wild-type) cannot be isolated as pure protein, no activity Homo sapiens
R115L exhibits wild-type kinetics, but is more stable than the wild-type Homo sapiens
S264L does not complement the ferrochelatase-deficient delta-hemH Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
 mesoporphyrin IX mutant M267I, heterodimer, room temperature Homo sapiens
0.0083
-
 Fe2+ mutant M267I, heterodimer, room temperature Homo sapiens
0.0092
-
 mesoporphyrin IX mutant Q139L, heterodimer, room temperature Homo sapiens
0.0094
-
 mesoporphyrin IX wild-type, heterodimer, room temperature Homo sapiens
0.0097
-
 mesoporphyrin IX mutant F260L, heterodimer, room temperature Homo sapiens
0.0114
-
 Fe2+ wild-type, heterodimer, room temperature Homo sapiens
0.0117
-
 Fe2+ mutant M267I, homodimer, room temperature Homo sapiens
0.0119
-
 Fe2+ wild-type, homodimer, room temperature Homo sapiens
0.0121
-
 mesoporphyrin IX wild-type, homodimer, room temperature Homo sapiens
0.0123
-
 mesoporphyrin IX mutant M267I, homodimer, room temperature Homo sapiens
0.0129
-
 mesoporphyrin IX mutant C236Y, heterodimer, room temperature Homo sapiens
0.0129
-
 mesoporphyrin IX mutant Y191H, homodimer, room temperature Homo sapiens
0.0138
-
 mesoporphyrin IX mutant S264L, heterodimer, room temperature Homo sapiens
0.0141
-
 Fe2+ mutant C236Y, homodimer, room temperature Homo sapiens
0.0146
-
 mesoporphyrin IX mutant F260L, homodimer, room temperature Homo sapiens
0.0172
-
 mesoporphyrin IX mutant Y191H, heterodimer, room temperature Homo sapiens
0.0179
-
 Fe2+ mutant C236Y, heterodimer, room temperature Homo sapiens
0.0182
-
 mesoporphyrin IX mutant P334L, homodimer, room temperature Homo sapiens
0.0219
-
 Fe2+ mutant Q139L, heterodimer, room temperature Homo sapiens
0.0224
-
 mesoporphyrin IX mutant K379N, homodimer, room temperature Homo sapiens
0.0232
-
 Fe2+ mutant K379N, heterodimer, room temperature Homo sapiens
0.0235
-
 Fe2+ mutant S264L, heterodimer, room temperature Homo sapiens
0.0251
-
 Fe2+ mutant Y191H, homodimer, room temperature Homo sapiens
0.0253
-
 mesoporphyrin IX mutant K379N, heterodimer, room temperature Homo sapiens
0.0262
-
 Fe2+ mutant Q139L, homodimer, room temperature Homo sapiens
0.0267
-
 mesoporphyrin IX mutant Q139L, homodimer, room temperature Homo sapiens
0.0302
-
 mesoporphyrin IX mutant C236Y, homodimer, room temperature Homo sapiens
0.038
-
 Fe2+ mutant Y191H, heterodimer, room temperature Homo sapiens
0.0441
-
 Fe2+ mutant F260L, heterodimer, room temperature Homo sapiens
0.0488
-
 Fe2+ mutant P334L, homodimer, room temperature Homo sapiens
0.0763
-
 Fe2+ mutant F260L, homodimer, room temperature Homo sapiens
0.1101
-
 Fe2+ mutant K379N, homodimer, room temperature Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 mutant C406S, dimer, dynamic light scattering Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin + Fe2+ Homo sapiens
-
 protoheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22830
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mesoporphyrin IX + ferrous ammonium sulfate room temperature Homo sapiens mesoheme + ammonium sulfate
-
 ?
protoporphyrin + Fe2+
-
 Homo sapiens protoheme + H+
-
 ?

Subunits

Subunits Comment Organism
dimer wild-type, gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
ferrochelatase
-
 Homo sapiens