Literature summary for 4.98.1.1 extracted from

Shi, Z.; Franco, R.; Haddad, R.; Shelnutt, J.A.; Ferreira, G.C.
The conserved active-site loop residues of ferrochelatase induce porphyrin conformational changes necessary for catalysis (2006), Biochemistry, 45, 2904-2912.

Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	protoporphyrin IX	wild-type, under strictly anaerobic conditions	Mus musculus
0.00251	-	protoporphyrin IX	mutant P255G, under strictly anaerobic conditions	Mus musculus
0.00265	-	protoporphyrin IX	mutant P255R, under strictly anaerobic conditions	Mus musculus
0.00684	-	protoporphyrin IX	mutant S249A/K250Q/V251C, under strictly anaerobic conditions	Mus musculus
0.01034	-	protoporphyrin IX	mutant Q248P/S249G/K250P/G252W, under strictly anaerobic conditions	Mus musculus
0.01125	-	protoporphyrin IX	mutant K250M/V251L/W256Y, under strictly anaerobic conditions	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protoporphyrin + Fe2+	Mus musculus	-	protoheme + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P22315	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protoporphyrin + Fe2+	-	Mus musculus	protoheme + H+	-	?
protoporphyrin IX + Fe2+	under strictly anaerobic conditions	Mus musculus	protoheme + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
ferrochelatase	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.051	-	protoporphyrin IX	mutant K250M/V251L/W256Y, under strictly anaerobic conditions	Mus musculus
0.068	-	protoporphyrin IX	wild-type, under strictly anaerobic conditions	Mus musculus
0.098	-	protoporphyrin IX	mutant P255G, under strictly anaerobic conditions	Mus musculus
0.13	-	protoporphyrin IX	mutant P255R, under strictly anaerobic conditions	Mus musculus
0.234	-	protoporphyrin IX	mutant Q248P/S249G/K250P/G252W, under strictly anaerobic conditions	Mus musculus
0.3	-	protoporphyrin IX	mutant S249A/K250Q/V251C, under strictly anaerobic conditions	Mus musculus

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Release 2023.1 (January 2023)