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Literature summary for 4.98.1.1 extracted from
- Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis (1994), Eur. J. Biochem., 220, 201-208.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
protoporphyrin | - |
Bacillus subtilis |  |
| 0.017 | - |
Zn2+ | - |
Bacillus subtilis | |
| 0.17 | - |
Cu2+ | - |
Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bacillus subtilis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin IX + Fe2+ | Bacillus subtilis | final step of heme biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin + Cu2+ | - |
Bacillus subtilis | Cu2+-protoporphyrin + H+ | - |
? | |
| protoporphyrin + Fe2+ | - |
Bacillus subtilis | protoheme + 2 H+ | - |
? | |
| protoporphyrin + Zn2+ | - |
Bacillus subtilis | Zn2+-protoporphyrin + H+ | - |
? | |
| protoporphyrin IX + Fe2+ | final step of heme biosynthesis | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE, gel filtration | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | 0.483 | protoporphyrin | - |
Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Bacillus subtilis |
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