General Stability | Organism |
---|---|
in absence of dithiothreitol or glycerol enzyme activity diminishes rapidly | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Rattus norvegicus | |
Cu2+ | - |
Rattus norvegicus | |
iodoacetamide | - |
Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus | |
Pb2+ | - |
Rattus norvegicus | |
sulfhydryl reagents | - |
Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0267 | - |
mesoporphyrin IX | - |
Rattus norvegicus | |
0.0285 | - |
protoporphyrin IX | - |
Rattus norvegicus | |
0.0331 | - |
Fe2+ | cosubstrate protoporphyrin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | inner membrane | Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibition | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
1 * 42000, SDS-PAGE | Rattus norvegicus |
240000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
glutathione, cysteine, dithiothreitol protect against autooxidation | Rattus norvegicus |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | lipoprotein, purified enzyme contains 0.5-1.9 mg fatty acids per mg protein | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
additional information | Rattus norvegicus |
stable for at 1 month, -25°C, 50% loss of activity, 4°C, 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesoporphyrin IX + Fe2+ | - |
Rattus norvegicus | mesoheme + 2 H+ | - |
? | |
protoporphyrin + Fe2+ | - |
Rattus norvegicus | protoheme + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 42000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9.5 | - |
Rattus norvegicus |